Vinogradov E, Radziejewska-Lebrecht J
Institute for Biological Sciences, National Research Council, Ottawa, Ont., Canada.
Carbohydr Res. 2000 Nov 3;329(2):351-7. doi: 10.1016/s0008-6215(00)00197-x.
The following structure of the lipid A-core region of the lipopolysaccharide (LPS) from Proteus mirabilis serotype O28 was determined using NMR, MS, and chemical analysis of the core oligosaccharide, obtained by mild acid hydrolysis of LPS, and of the products of alkaline deacylation of the LPS: carbohydrate sequence [see text] where S = beta-GalALys (amide of beta-D-galactopyranosyluronic acid with the alpha-amino group of L-lysine) or beta-GalALys4PEtN are present in the ratio of approximately 1:1. beta-GalA and Ara4N (indicated by bold italics) are present in non stoichometric amount. All sugars are present in the pyranose form and all except L-Ara4N have the D configuration.
利用核磁共振（NMR）、质谱（MS）以及对通过温和酸水解脂多糖（LPS）得到的核心寡糖和LPS碱性脱酰产物进行化学分析，确定了奇异变形杆菌O28血清型脂多糖（LPS）的脂质A核心区域的以下结构：碳水化合物序列[见正文]，其中S = β-GalALys（β-D-吡喃半乳糖醛酸与L-赖氨酸α-氨基的酰胺）或β-GalALys4PEtN的比例约为1:1。β-GalA和Ara4N（用粗斜体表示）的含量不呈化学计量。所有糖类均以吡喃糖形式存在，除L-Ara4N外均为D构型。
