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sp. nov., isolated from roots of .

作者信息

Li Yan-Qiong, Li Li, Fu Yu-Song, Cui Zhao-Qiong, Duan Yan-Qing, Salam Nimaichand, Guo Jian-Wei, Chen Wei, Li Wen-Jun

机构信息

Kunming Medical University Haiyuan College,Kunming, 650106,PR China.

Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences,Urumqi, 830011,PR China.

出版信息

Int J Syst Evol Microbiol. 2016 Mar;66(3):1287-1292. doi: 10.1099/ijsem.0.000876. Epub 2016 Jan 5.

Abstract

A Gram-stain-positive, aerobic, rod-shaped, non-motile actinomycete strain, designated EGI 60007, was isolated from healthy roots of F. collected from Yili County, Xinjiang Province, north-west China. A polyphasic approach was applied to study the taxonomic position of the new isolate. The 16S rRNA gene sequence of strain EGI 60007 had highest similarities with members of the genus , including CAU 59 (96.98 % 16S rRNA gene sequence similarity), DSM 20419 (96.43 %) and THG-MD12 (96.14 %). The neighbour-joining phylogenetic tree based on 16S rRNA gene sequences showed that strain EGI 60007 clustered with members of the genus , and formed a distinct clade with CAU 59. The polar lipids detected for strain EGI 60007 were phosphatidylglycerol, diphosphatidylglycerol, one unidentified glycolipid and one unidentified lipid. The DNA G+C content was determined to be 63.3 mol%. The chemotaxonomic features of strain EGI 60007 showed typical characteristics of the genus , with MK-9 as the respiratory quinone, 2,4-diaminobutyric acid as the diamino acid in the peptidoglycan, and anteiso-C, anteiso-C and iso-C as the major fatty acids. The sugars of whole-cell hydrolysates were mainly mannose, rhamnose, ribose and glucose, and a minor amount of xylose. Based on the results of the phylogentic analysis supported by morphological, physiological, chemotaxonomic and other differentiating phenotypic characteristics, strain EGI 60007 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is EGI 60007 ( = CGMCC 1.15081 = KCTC 39112 = DSM 29943).

摘要

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