钟氏假单胞菌，从活性污泥中分离得到的一株新的假单胞菌。
Pseudoclavibacter chungangensis sp. nov., isolated from activated sludge.
机构信息
Department of Microbiology, Chung-Ang University College of Medicine, 221 Heukseok-dong, Dongjak-gu, Seoul 156-756, Republic of Korea.
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejeon, Republic of Korea.
出版信息
Int J Syst Evol Microbiol. 2010 Jul;60(Pt 7):1672-1677. doi: 10.1099/ijs.0.015552-0. Epub 2009 Sep 4.
The taxonomic position of a Gram-positive, non-spore-forming strain, designated CAU 59(T), from activated sludge was investigated. Colony morphology, biochemical tests and chemotaxonomic investigations revealed that strain CAU 59(T) possessed the characteristics of the genus Pseudoclavibacter. Comparative 16S rRNA gene sequence analysis showed sequence divergence values between strain CAU 59(T) and other described Pseudoclavibacter species of more than 3.6 %, and the strain formed a hitherto-unknown subline within the genus Pseudoclavibacter. DNA-DNA hybridization studies showed that strain CAU 59(T) displayed 20.9 % relatedness to its closest phylogenetic neighbour, Pseudoclavibacter helvolus DSM 20419(T). The DNA G+C content was 66.2 mol%. The phenotypic, chemotaxonomic and genotypic data indicated that strain CAU 59(T) represents a novel species of the genus Pseudoclavibacter, for which the name Pseudoclavibacter chungangensis sp. nov. is proposed. The type strain is CAU 59(T) (=KCTC 22691(T) =CCUG 58142(T)).
从活性污泥中分离到一株革兰氏阳性、非芽孢形成的菌株，命名为 CAU 59(T)。对其进行了表型、生理生化和化学分类学研究。结果表明，CAU 59(T) 菌株具有假克利夫巴斯德菌属的特征。16S rRNA 基因序列分析结果显示，CAU 59(T) 菌株与其他已描述的假克利夫巴斯德菌属的种之间的序列差异超过 3.6%，并且在假克利夫巴斯德菌属内形成了一个未知的亚群。DNA-DNA 杂交研究表明，CAU 59(T) 菌株与最近的亲缘种 Helvolibacter helvolus DSM 20419(T) 的同源性为 20.9%。该菌株的 DNA G+C 含量为 66.2 mol%。综合表型、生理生化和遗传学特征，CAU 59(T) 菌株代表了假克利夫巴斯德菌属的一个新种，命名为 Chungangensis 假克利夫巴斯德菌(Pseudoclavibacter chungangensis sp. nov.)。模式菌株为 CAU 59(T)(=KCTC 22691(T)=CCUG 58142(T))。
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