Youngleson J S, Jones W A, Jones D T, Woods D R
Department of Microbiology, University of Cape Town, South Africa.
Gene. 1989 May 30;78(2):355-64. doi: 10.1016/0378-1119(89)90238-2.
The nucleotide sequence of a 2081-bp fragment of Clostridium acetobutylicum DNA containing the adh1 gene was determined. The butanol dehydrogenase gene is referred to as the adh1 gene since it was shown to have activity using butanol and ethanol as substrates. The adh1 gene consisted of 1164 bp and encoded an alcohol dehydrogenase (ADH) enzyme of 388 aa residues with an Mr of 43,274. The adh1 gene was separated from an upstream open reading frame by an intergenic region of 354 bp. No promoter consensus sequences were identified in the intergenic upstream region and the adh1 gene did not appear to be expressed off its own promoter in Escherichia coli. Three separate types of ADH have been recognized. The ADH1 from C. acetobutylicum exhibited 39% homology with the Fe-containing ADH2 from Zymomonas mobilis and 37% homology with the ADH4 from Saccharomyces cerevisiae, but showed little or no homology with the other characterised types of ADH.
测定了丙酮丁醇梭菌（Clostridium acetobutylicum）DNA中包含adh1基因的一个2081 bp片段的核苷酸序列。由于已证明该丁醇脱氢酶基因以丁醇和乙醇为底物具有活性，因此被称为adh1基因。adh1基因由1164 bp组成，编码一种含388个氨基酸残基、Mr为43274的醇脱氢酶（ADH）。adh1基因与上游开放阅读框之间被一个354 bp的基因间区域隔开。在基因间上游区域未鉴定到启动子共有序列，并且adh1基因在大肠杆菌中似乎不是由其自身的启动子表达的。已识别出三种不同类型的ADH。丙酮丁醇梭菌的ADH1与运动发酵单胞菌（Zymomonas mobilis）含Fe的ADH2具有39%的同源性，与酿酒酵母（Saccharomyces cerevisiae）的ADH4具有37%的同源性，但与其他已鉴定的ADH类型几乎没有同源性。
