巨大脱硫弧菌中一种对氧敏感的、依赖NAD的醇脱氢酶的纯化及特性分析
Purification and characterization of an oxygen-labile, NAD-dependent alcohol dehydrogenase from Desulfovibrio gigas.
作者信息
Hensgens C M, Vonck J, Van Beeumen J, van Bruggen E F, Hansen T A
机构信息
Department of Microbiology, University of Groningen, Haren, The Netherlands.
出版信息
J Bacteriol. 1993 May;175(10):2859-63. doi: 10.1128/jb.175.10.2859-2863.1993.
A NAD-dependent, oxygen-labile alcohol dehydrogenase was purified from Desulfovibrio gigas. It was decameric, with subunits of M(r) 43,000. The best substrates were ethanol (Km, 0.15 mM) and 1-propanol (Km, 0.28 mM). N-terminal amino acid sequence analysis showed that the enzyme belongs to the same family of alcohol dehydrogenases as Zymomonas mobilis ADH2 and Bacillus methanolicus MDH.
从巨大脱硫弧菌中纯化出一种依赖烟酰胺腺嘌呤二核苷酸（NAD）且对氧不稳定的醇脱氢酶。它是十聚体，亚基的相对分子质量（M(r)）为43,000。最佳底物是乙醇（Km为0.15 mM）和1-丙醇（Km为0.28 mM）。N端氨基酸序列分析表明，该酶与运动发酵单胞菌ADH2和甲醇芽孢杆菌MDH属于同一醇脱氢酶家族。
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