Torres Leticia, Hu E, Tiersch Terrence R
Reprod Fertil Dev. 2016 Jan 7. doi: 10.1071/RD15388.
Cryopreservation in aquatic species in general has been constrained to research activities for more than 60 years. Although the need for application and commercialisation pathways has become clear, the lack of comprehensive quality assurance and quality control programs has impeded the progress of the field, delaying the establishment of germplasm repositories and commercial-scale applications. In this review we focus on the opportunities for standardisation in the practices involved in the four main stages of the cryopreservation process: (1) source, housing and conditioning of fish; (2) sample collection and preparation; (3) freezing and cryogenic storage of samples; and (4) egg collection and use of thawed sperm samples. In addition, we introduce some key factors that would assist the transition to commercial-scale, high-throughput application.
总体而言，60多年来，水生物种的冷冻保存一直局限于研究活动。尽管应用和商业化途径的需求已变得明确，但缺乏全面的质量保证和质量控制计划阻碍了该领域的发展，推迟了种质库的建立和商业规模应用。在本综述中，我们重点关注冷冻保存过程四个主要阶段所涉及实践的标准化机会：（1）鱼类的来源、饲养和驯化；（2）样本采集和制备；（3）样本的冷冻和低温保存；（4）卵子采集和解冻精子样本的使用。此外，我们还介绍了一些有助于向商业规模、高通量应用过渡的关键因素。
