Childress William M, Caffey Rex H, Tiersch Terrence R
Aquatic Germplasm and Genetic Resources Center, School of Renewable Natural Resources, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70820 USA.
Center for Natural Resource Economics and Policy, Department of Agricultural Economics and Agribusiness, Louisiana State University Agricultural Center, Baton Rouge, Louisiana 70803, USA.
J World Aquac Soc. 2018 Oct;49(5):805-826. doi: 10.1111/jwas.12525. Epub 2018 Apr 16.
Although aquatic species cryopreservation protocols have been studied around the world over the past 60 yr., germplasm repository development efforts and commercialization have begun only recently. The goal of this project was to develop a self-contained mobile laboratory for on-site high-throughput cryopreservation of aquatic species. The objectives of this study were to: (1) identify how a mobile laboratory would function in different operational scenarios, (2) customize an enclosed cargo trailer to function as a mobile laboratory, (3) evaluate the laboratory layout and ability of cryopreservation equipment to operate from generator power, and (4) document the investment costs for private and public groups to integrate a mobile laboratory into an existing cryopreservation facility at three levels of automation and estimate the total cost per trip based on hypothetical assumptions for two scenarios (aquaculture production and repository development). There were three operational designs identified for the mobile laboratory: (1) self-contained work inside the unit using generator power, (2) work inside the unit using external facility power, and (3) using the equipment inside of a host facility. The investment costs for a base-level mobile laboratory ranged between US$5670 and US$5787 for private groups and between US$5208 and US$5315 for public groups. With the addition of a range of automated processing equipment, total investment costs ranged from US$13,616 to US$103,529 for private groups and US$12,494 to US$94,891 for public groups. The total cost per trip to cryopreserve sperm of 59 blue catfish, males to produce 6300 0.5-mL French straws was estimated to range from US$6089 to US$14,633 for private and between US$5703 and US$16,938 for public groups depending on the level of automation. Total cost per trip to cryopreserve sperm of 500 males of five different species in the genus to produce 641 0.25-mL French straws was estimated to range from US$6653 to US$7640 for private and US$7582 to US$8088 for public groups depending on level of automation. Overall, a commercial-scale mobile laboratory was developed that can assist current germplasm activities and support future repository and industry development, and the layout information provided can help others to design and build comparable units.
尽管在过去60年里世界各地都在研究水生物种的冷冻保存方案，但种质库的开发工作和商业化直到最近才开始。该项目的目标是开发一个独立的移动实验室，用于现场高通量冷冻保存水生物种。本研究的目的是：（1）确定移动实验室在不同操作场景下的功能，（2）定制一个封闭式货运拖车使其作为移动实验室使用，（3）评估实验室布局以及冷冻保存设备使用发电机供电的运行能力，（4）记录私人和公共团体将移动实验室整合到现有冷冻保存设施中的投资成本，分为三个自动化级别，并根据两种场景（水产养殖生产和种质库开发）的假设估算每次行程的总成本。为移动实验室确定了三种操作设计：（1）使用发电机供电在设备内部独立工作，（2）使用外部设施电源在设备内部工作，（3）在宿主设施内使用设备。基础级移动实验室的投资成本，私人团体在5670美元至5787美元之间，公共团体在5208美元至5315美元之间。加上一系列自动化处理设备后，私人团体的总投资成本在13,616美元至103,529美元之间，公共团体在12,494美元至94,891美元之间。根据自动化程度，冷冻保存59条雄性蓝鲶的精子以生产6300根0.5毫升法式细管的每次行程总成本，估计私人团体在6089美元至14,633美元之间，公共团体在5703美元至16,938美元之间。根据自动化程度，冷冻保存500条属于五个不同物种的雄性精子以生产641根0.25毫升法式细管的每次行程总成本，估计私人团体在6653美元至7640美元之间，公共团体在7582美元至8088美元之间。总体而言，开发了一个商业规模的移动实验室，它可以协助当前的种质活动并支持未来的种质库和行业发展，所提供的布局信息可以帮助其他人设计和建造类似的设备。
