Piechal Agnieszka, Blecharz-Klin Kamilla, Pyrzanowska Justyna, Widy-Tyszkiewicz Ewa
Department of Experimental and Clinical Pharmacology, Centre for Preclinical Research and Technology CePT, Medical University of Warsaw, Banacha 1b, 02-097, Warsaw, Poland.
Biol Trace Elem Res. 2016 Aug;172(2):408-418. doi: 10.1007/s12011-015-0597-8. Epub 2016 Jan 6.
Animal brain contains a significant amount of zinc, which is a cofactor for more than 300 enzymes. Moreover, it provides the basis for functioning of more than 2000 transcription factors, and it is necessary for memory formation and learning processes in the brain. The aim of this study was to investigate the effect of zinc supplementation on behavior in 3-month-old rats. For this purpose, the Morris water maze paradigm, hole-board, and T-maze were used. Wistar rats received a solution of ZnSO4 in drinking water at the doses of 16 mg/kg (Zn16 group) and 32 mg/kg (Zn32 group). In rats pretreated with the lower dose of zinc, the improvement of the mean escape latency was observed in comparison to the control group and Zn32 group. During memory task, both ZnSO4-supplemented groups showed an increase in crossings over the previous platform position. Furthermore, the exploratory activity in Zn16 group was improved in comparison to Zn32 and control group. In the brains of zinc-supplemented rats, we observed the higher content of zinc, both in the hippocampus and the prefrontal cortex. Hippocampal zinc level correlated positively with the mean annulus crossings of the Zn16 group during the probe trial. These findings show that the long-term administration of ZnS04 can improve learning, spatial memory, and exploratory activity in rats. Graphical Abstract Improvement of spatial learning, memory, and exploratory behavior.
动物大脑含有大量锌，锌是300多种酶的辅助因子。此外，它为2000多种转录因子的功能发挥提供了基础，并且是大脑中记忆形成和学习过程所必需的。本研究的目的是调查补充锌对3月龄大鼠行为的影响。为此，采用了莫里斯水迷宫范式、洞板试验和T迷宫试验。Wistar大鼠饮用含硫酸锌溶液，剂量分别为16毫克/千克（锌16组）和32毫克/千克（锌32组）。与对照组和锌32组相比，低剂量锌预处理的大鼠平均逃避潜伏期有所改善。在记忆任务期间，两个补充硫酸锌的组在前平台位置的穿越次数均增加。此外，与锌32组和对照组相比，锌16组的探索活动有所改善。在补充锌的大鼠大脑中，我们观察到海马体和前额叶皮质中的锌含量均较高。在探针试验期间，海马体锌水平与锌16组的平均环道穿越次数呈正相关。这些发现表明，长期给予硫酸锌可以改善大鼠的学习、空间记忆和探索活动。图形摘要 空间学习、记忆和探索行为的改善。
