Shanghai Academy of Agricultural Sciences, 201106 Shanghai, China.
Mol Biol Rep. 2010 Jun;37(5):2485-92. doi: 10.1007/s11033-009-9763-4. Epub 2009 Aug 23.
Chinese cabbage (Brassica rapa subsp. pekinensis) is among the most important vegetables and is widely cultivated in world. Genes in the AP2/ERF family encode transcriptional regulators that serve a variety of functions in the plants. Expressed sequence tags (ESTs) are created by partially sequencing randomly isolated gene transcripts and have proved valuable in molecular biology. Starting from the database with 142 947 ESTs of B. rapa, 62 putative AP2/ERF family genes were identified by in silico cloning using the conserved AP2/ERF domain amino acid sequence of Arabidopsis thaliana as a probe. Based on the number of AP2/ERF domains and functions of the genes, the AP2/ERF transcription factors from B. rapa were classified into four subfamilies (DREB, ERF, AP2 and RAV). Using large-scale available EST information as a source of expression data for digital expression profiling, differentially detected genes were identified among diverse plant tissues. Roots contained the largest number of transcripts of the AP2/ERF family genes, followed by leaves and seeds. Only a few of the 62 AP2/ERF family genes were detected in all tissues: most were detected only in some tissues but not in others. The maximum detected was that of BraERF-B2-5, and it was recorded from seed tissue.
白菜（ Brassica rapa subsp. pekinensis ）是最重要的蔬菜之一，在世界范围内广泛种植。AP2/ERF 家族的基因编码转录调节因子，在植物中具有多种功能。表达序列标签（ESTs）是通过部分测序随机分离的基因转录本而创建的，已被证明在分子生物学中具有重要价值。从包含 142947 个 B. rapa ESTs 的数据库开始，通过使用拟南芥（ Arabidopsis thaliana ）的保守 AP2/ERF 结构域氨基酸序列作为探针，通过计算机克隆鉴定了 62 个假定的 AP2/ERF 家族基因。根据 AP2/ERF 结构域的数量和基因的功能，B. rapa 的 AP2/ERF 转录因子分为四个亚家族（DREB、ERF、AP2 和 RAV）。利用大规模可用的 EST 信息作为数字表达谱分析的表达数据来源，在不同的植物组织中鉴定出差异表达的基因。根组织中含有最多的 AP2/ERF 家族基因转录本，其次是叶和种子。在所有组织中仅检测到 62 个 AP2/ERF 家族基因中的少数几个：大多数仅在某些组织中检测到，而在其他组织中则未检测到。检测到的最多的是 BraERF-B2-5，它是从种子组织中记录的。
