Karadag Hasan, Ozhan Fadil
Department of Chemistry, Faculty of Science and Letters, University of Adiyaman , Adiyaman , Turkey.
Biotechnol Biotechnol Equip. 2015 Jan 2;29(1):40-44. doi: 10.1080/13102818.2014.992740. Epub 2015 Jan 7.
This study investigated if the use of the pesticides cyprodinil and fludioxonil produced an inhibitory effect on the bovine liver catalase (CAT) activity. It was documented that the activity of the enzyme decreased with increasing concentrations of cyprodinil and fludioxonil from 0 to 500 ppm. At pesticide concentrations of 250 and 500 ppm, the activity of CAT remained unchanged and passed to a steady state. The exposure to cyprodinil in concentrations of 10, 50, 100, 250 and 500 ppm, led to a decrease in the per cent of the CAT enzyme activity calculated as 45.4, 68.0, 73.0, 77.8 and 77.4, respectively. Similarly, the exposure to fludioxonil in concentrations of 10, 50, 100, 250 and 500 ppm, produced the following percentage decrease in the CAT enzyme activity: 20.0, 30.8, 42.8, 46.3 and 45.9, respectively. Cyprodinil inhibited CAT competitively, whereas the mechanism of fludioxonil inhibition over the enzyme was non-competitive.
本研究调查了杀菌剂嘧菌环胺和咯菌腈的使用是否会对牛肝过氧化氢酶（CAT）的活性产生抑制作用。据记载，随着嘧菌环胺和咯菌腈浓度从0增加到500 ppm，该酶的活性降低。在250和500 ppm的农药浓度下，CAT的活性保持不变并进入稳定状态。暴露于浓度为10、50、100、250和500 ppm的嘧菌环胺中，导致CAT酶活性百分比分别下降45.4%、68.0%、73.0%、77.8%和77.4%。同样，暴露于浓度为10、50、100、250和500 ppm的咯菌腈中，CAT酶活性的下降百分比分别为：20.0%、30.8%、42.8%、46.3%和45.9%。嘧菌环胺竞争性抑制CAT，而咯菌腈对该酶的抑制机制是非竞争性的。
