Raţiu Cristian Adrian, Cavalu Simona Daniela, Miclăuş Viorel, Rus Vasile, Lăzărescu Grigore Ion
Department of Preclinical Sciences, Faculty of Medicine and Pharmacy, University of Oradea, Romania;
Rom J Morphol Embryol. 2015;56(4):1455-60.
The investigation of desired optimal interface between bone and dental or orthopedic implants, and whether a newly developed material conforms to the requirements of biocompatibility and mechanical stability, are important and rigorous procedures as an essential step prior to clinical trials. The present study proposes the investigation of a novel ceramic implant in terms of biocompatibility, osseointegration and bone response, by an experimental study using a small animal model (rabbit). Radiological examination after six weeks post-surgery highlighted the stability and well integration of the implant, without fibrous tissue or other undesirable complications. The histological analyses highlighted the morphological details of the new tissue, which was well vascularized throughout its thickness. The new bone formation ensured not only the coverage of the implant hole, but also the continuity with the adjacent bone. The chemical stability is suggested by the XRD (X-rays diffraction) pattern, as the implant did not showed erosion marks at the surface, not even discrete ones. Moreover, the XRD pattern recorded on the surface of femoral bone showed the fingerprints of hydroxyapatite indicating that the new bone covered the surface of the implanted area. The qualitative and quantitative aspects of the new bone were highlighted through light microscopy and SEM÷EDX (scanning electron microscopy÷energy dispersive X-rays), especially the lamellar architecture of the new bone at the contact area with the implant, six weeks after insertion. The CA÷P ratio was evaluated, which is a valuable indicator in qualitative assessment of the osseous tissue.
研究骨与牙科或骨科植入物之间理想的最佳界面，以及一种新开发的材料是否符合生物相容性和机械稳定性的要求，是临床试验前至关重要且严格的程序。本研究通过使用小动物模型（兔子）的实验研究，对一种新型陶瓷植入物的生物相容性、骨整合和骨反应进行研究。术后六周的放射学检查突出了植入物的稳定性和良好整合性，没有纤维组织或其他不良并发症。组织学分析突出了新组织的形态细节，其在整个厚度上都有良好的血管化。新骨形成不仅确保了植入孔的覆盖，还确保了与相邻骨的连续性。XRD（X射线衍射）图谱表明了化学稳定性，因为植入物表面没有显示出侵蚀痕迹，甚至没有离散的痕迹。此外，在股骨表面记录的XRD图谱显示了羟基磷灰石的指纹特征，表明新骨覆盖了植入区域的表面。通过光学显微镜和SEM÷EDX（扫描电子显微镜÷能量色散X射线）突出了新骨的定性和定量方面，特别是植入六周后在与植入物接触区域新骨的层状结构。评估了Ca÷P比值，这是骨组织定性评估中的一个有价值的指标。
