FZP在水稻穗分支和小穗形成决定中的调控作用。

Regulatory role of FZP in the determination of panicle branching and spikelet formation in rice.

作者信息

Bai Xufeng, Huang Yong, Mao Donghai, Wen Mi, Zhang Li, Xing Yongzhong

机构信息

National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research (Wuhan), Huazhong Agricultural University, Wuhan 430070, China.

Hubei Collaborative Innovation Center for Grain Industry, Yangtze University, Jingzhou 434025, China.

出版信息

Sci Rep. 2016 Jan 8;6:19022. doi: 10.1038/srep19022.

FRIZZLE PANICLE (FZP) and RFL/ABERRANT PANICLE ORGANIZATION 2 (APO2) play important roles in regulating the ABCDE floral organ identity genes. However, the relationships among FZP and these floral identity genes in the regulation of panicle formation remain unclear. Here, we used the novel mutant fzp-11, wild-type and FZP-overexpressing plants to compare the expression of these genes during panicle development by real-time PCR and in situ hybridization. The results indicate that FZP is a major negative regulator of RFL/APO2 and determines the transition from panicle branching to spikelet formation. Moreover, overexpression of FZP severely represses axillary meristem formation in both the vegetative and reproductive phases and the outgrowth of secondary branches in panicle. FZP overexpression positively regulates the expression of a subset of the class B genes, AGL6 genes (OsMADS6 and OsMADS17) as well as class E genes (OsMADS1, OsMADS7 and OsMADS8) in floral meristem (FM). Thus, it suggested that FZP could specify floral organ identity by regulating the related OsMADS-box genes.

卷曲穗（FZP）和RFL/异常穗器官组织2（APO2）在调控ABCDE花器官特征基因方面发挥着重要作用。然而，FZP与这些花器官特征基因在穗形成调控中的关系仍不清楚。在此，我们使用新型突变体fzp-11、野生型和过表达FZP的植株，通过实时定量PCR和原位杂交比较这些基因在穗发育过程中的表达情况。结果表明，FZP是RFL/APO2的主要负调控因子，并决定了从穗分支到小穗形成的转变。此外，FZP的过表达严重抑制了营养期和生殖期腋生分生组织的形成以及穗中二级分支的生长。FZP过表达正向调控花分生组织（FM）中B类基因的一个子集、AGL6基因（OsMADS6和OsMADS17）以及E类基因（OsMADS1、OsMADS7和OsMADS8）的表达。因此，表明FZP可能通过调控相关的OsMADS-box基因来确定花器官特征。