Liu Haiping, Wang Jianye, Wang Haiyu, Tang Ning, Li Yunfei, Zhang Yan, Hao Tianyu
Reproductive Medicine Centre of Jinan Military General Hospital, Jinan, P.R. China.
Reproductive Medicine Centre of Jinan Military General Hospital, Jinan, P.R. China
Ann Clin Biochem. 2016 Sep;53(Pt 5):599-605. doi: 10.1177/0004563215626458. Epub 2016 Jan 7.
Enzyme matrix metalloproteinase-9 is a member of the matrix metalloproteinase family, which is critical to normal tissue remodelling during embryogenesis and wound healing. In patients with endometriosis, increased expression and activity of matrix metalloproteinase-9 have been observed in ectopic endometrium, but the plasma and peritoneal fluid concentrations of matrix metalloproteinase-9 in patients with endometriosis and their relation to disease severity have not been clear. The aim of the study was to investigate the concentrations of matrix metalloproteinase-9 in plasma and peritoneal fluid of patients with endometriosis.
A prospective case-control study was conducted in Jinan Military General Hospital between January 2010 and December 2013. Fifty patients with proven endometriosis and 26 endometriosis-free controls were enrolled in this study. Patients with endometriosis were evaluated and divided into moderate/severe endometriosis group (stage I-II, n = 26) and minimal/mild endometriosis group (stage III-IV, n = 24) according to the revised criteria of the American Society for Reproductive Medicine. Blood samples and peritoneal fluid were obtained from both patients and controls. Matrix metalloproteinase-9 was measured using enzyme-linked immunosorbent assay in plasma and peritoneal fluid. The concentration of matrix metalloproteinase-9 between different groups was compared and its correlation to disease severity was analysed.
Plasma and peritoneal fluid concentrations of matrix metalloproteinase-9 in patients with endometriosis were higher than that in controls. In addition, those patients with moderate/severe endometriosis had significantly higher plasma and peritoneal fluid concentrations of matrix metalloproteinase-9 compared to those with minimal/mild endometriosis. Matrix metalloproteinase-9 concentrations in plasma and peritoneal fluid were both positively correlated with severity of endometriosis and plasma matrix metalloproteinase-9 concentrations had a positive correlation with peritoneal fluid matrix metalloproteinase-9 concentrations in patients with endometriosis.
Increased concentrations of plasma and peritoneal fluid concentrations of matrix metalloproteinase-9 appear to be associated with disease severity of endometriosis and may serve as an alternative biomarker to determine disease severity of endometriosis.
基质金属蛋白酶-9是基质金属蛋白酶家族的成员，对胚胎发育和伤口愈合过程中的正常组织重塑至关重要。在子宫内膜异位症患者中，异位内膜中基质金属蛋白酶-9的表达和活性增加，但子宫内膜异位症患者血浆和腹腔液中基质金属蛋白酶-9的浓度及其与疾病严重程度的关系尚不清楚。本研究的目的是调查子宫内膜异位症患者血浆和腹腔液中基质金属蛋白酶-9的浓度。
2010年1月至2013年12月在济南军区总医院进行了一项前瞻性病例对照研究。本研究纳入了50例经证实的子宫内膜异位症患者和26例无子宫内膜异位症的对照。根据美国生殖医学学会修订标准，对子宫内膜异位症患者进行评估，并分为中度/重度子宫内膜异位症组（I-II期，n = 26）和轻度/轻度子宫内膜异位症组（III-IV期，n = 24）。从患者和对照中获取血样和腹腔液。采用酶联免疫吸附测定法测定血浆和腹腔液中的基质金属蛋白酶-9。比较不同组间基质金属蛋白酶-9的浓度，并分析其与疾病严重程度的相关性。
子宫内膜异位症患者血浆和腹腔液中基质金属蛋白酶-9的浓度高于对照组。此外，与轻度/轻度子宫内膜异位症患者相比，中度/重度子宫内膜异位症患者血浆和腹腔液中基质金属蛋白酶-9的浓度显著更高。子宫内膜异位症患者血浆和腹腔液中基质金属蛋白酶-9的浓度均与子宫内膜异位症的严重程度呈正相关，且血浆基质金属蛋白酶-9的浓度与腹腔液基质金属蛋白酶-9的浓度呈正相关。
血浆和腹腔液中基质金属蛋白酶-9浓度的增加似乎与子宫内膜异位症的疾病严重程度相关，可能作为确定子宫内膜异位症疾病严重程度的替代生物标志物。
