Barbe Adrian Mykhailovych, Berbets Andrii Mykolaiovych, Davydenko Igor Sviatoslavovych, Koval Halyna Danylivna, Yuzko Viktoriia Oleksandrivna, Yuzko Oleksandr Mykhailovych
Department of Obstetrics and Gynecology, Bukovinian State Medical University, Chernivtsi, Ukraine.
Department of Pathologic Anatomy, Bukovinian State Medical University, Chernivtsi, Ukraine.
J Med Life. 2020 Jul-Sep;13(3):314-320. doi: 10.25122/jml-2020-0117.
Endometriosis is a chronic benign hormone-dependent condition when the endometrial tissue, identical with the endometrium by its morphological and functional properties, grows outside the borders of the uterine mucous membrane. Recent studies have pointed to the possible role of matrix metalloproteinases (MMPs) in the pathogenesis of endometriosis. We suggested a hypothesis that increased expression of MMPs activity in eutopic and ectopic endometrium of patients with endometriosis might correlate with the presence of endometriotic lesions. The aim of the study was to evaluate the level of MMP-2 and MMP-9 expression in the ectopic endometrium of women with visible endometriotic lesions and eutopic endometrium in patients with no signs of endometriosis. The study was conducted on 43 patients. They were divided into two groups. Group 1 included 31 patients with peritoneal/ovarian endometriosis who had undergone laparoscopy and hysteroscopy. Group 2 consisted of 12 patients with leiomyoma, endometrial polyps or relatively healthy patients who had undergone hysterectomy or polypectomy and endometrial curettage. This study showed statistically higher expression of MMP-2 (1.7783 ± 0.22 immunohistochemistry (IHC) optical density score compared to the control group - 1.41± 0.34, p = 0.0017) and MMP-9 (1.352 ± 0.067 versus 1.85 ± 0.26 in the control group, p = 0.001) in ectopic and eutopic endometrium samples from patients with endometriosis compared to samples taken from patients without endometriosis. A strong correlation between expression of the above-mentioned MMPs (r=0.74 for MMP-2 and r=0.88 for MMP-9) in ectopic and eutopic endometrium might be of promising diagnostic value.
子宫内膜异位症是一种慢性良性激素依赖性疾病，即形态和功能特性与子宫内膜相同的内膜组织在子宫黏膜边界以外生长。最近的研究指出基质金属蛋白酶（MMPs）在子宫内膜异位症发病机制中可能发挥的作用。我们提出一个假说，即子宫内膜异位症患者在位和异位内膜中MMPs活性表达增加可能与子宫内膜异位病变的存在相关。本研究的目的是评估有可见子宫内膜异位病变的女性异位内膜以及无子宫内膜异位症迹象患者的在位内膜中MMP-2和MMP-9的表达水平。该研究对43例患者进行。他们被分为两组。第1组包括31例接受腹腔镜检查和宫腔镜检查的腹膜/卵巢子宫内膜异位症患者。第2组由12例患有平滑肌瘤、子宫内膜息肉的患者或相对健康且接受过子宫切除术、息肉切除术和子宫内膜刮宫术的患者组成。本研究显示，与无子宫内膜异位症患者的样本相比，子宫内膜异位症患者异位和在位内膜样本中MMP-2（免疫组织化学（IHC）光密度评分1.7783±0.22，对照组为1.41±0.34，p = 0.0017）和MMP-9（分别为1.352±0.067和1.85±0.26，p = 0.001）的表达在统计学上更高。上述MMPs在异位和在位内膜中的表达之间存在强相关性（MMP-2的r = 0.74，MMP-9的r = 0.88），这可能具有有前景的诊断价值。
