Neves A R, Nunes C, Amenitsch H, Reis S
UCIBIO, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto, Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
Soft Matter. 2016 Feb 21;12(7):2118-26. doi: 10.1039/c5sm02905h. Epub 2016 Jan 8.
Resveratrol is a natural active compound which has been attracting increasing interest due to its several pharmacological effects in cancer prevention, cardiovascular protection and treatment of neurodegenerative disorders and diabetes. The current work investigates how resveratrol affects membrane order and structure, gathering information determined by X-ray scattering analysis, derivative spectrophotometry, fluorescence quenching and fluorescence anisotropy studies. The results indicate that resveratrol is able to be incorporated into DMPC liposome model systems, either fluidizing or stiffening the bilayer, which largely depends on the membrane fluidity state. These findings suggest that the effects of resveratrol resemble cholesterol action on biological membranes, thereby contributing to the regulation of cell membrane structure and fluidity, which may influence the activity of transmembrane proteins and hence control the cell signaling pathways. The regulation of a number of cellular functions, thus may contribute to the pharmacological and therapeutic activities of this compound, explaining its pleiotropic action.
白藜芦醇是一种天然活性化合物，因其在癌症预防、心血管保护以及神经退行性疾病和糖尿病治疗方面的多种药理作用而受到越来越多的关注。目前的研究工作探究了白藜芦醇如何影响膜的有序性和结构，收集了通过X射线散射分析、导数分光光度法、荧光猝灭和荧光各向异性研究确定的信息。结果表明，白藜芦醇能够掺入二肉豆蔻酰磷脂酰胆碱（DMPC）脂质体模型系统中，使双层膜流化或硬化，这在很大程度上取决于膜的流动性状态。这些发现表明，白藜芦醇的作用类似于胆固醇对生物膜的作用，从而有助于调节细胞膜的结构和流动性，这可能会影响跨膜蛋白的活性，进而控制细胞信号通路。因此，对许多细胞功能的调节可能有助于该化合物的药理和治疗活性，解释了其多效性作用。
