Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.
Anal Sci. 2023 May;39(5):705-712. doi: 10.1007/s44211-023-00278-9. Epub 2023 Feb 4.
Reactive oxygen species are known to be involved in various diseases, and antioxidant ingredients are expected to essentially prevent diseases and contribute to improving health. However, antioxidants are easily degraded by enzymes before being absorbed in the intestine, so a means of transport that prevents their degradation in the body is necessary. Exosomes, which play an important role in communication between individual cells, have attracted attention as a new transport carrier of miRNA and DNA, but not yet fully exploited in food research. More recently, exosomes extracted from bovine milk began to be widely used as a cost-effective transport carrier not in clinical medicine but also in functional food materials. To develop practical applications as carriers for functional foods, systematic studies are necessary to clarify the introduction efficiency and the properties of encapsulated substances. In this study, we applied electroporation and incubation to encapsulate antioxidants into the exosomes and studied the encapsulation efficiency into the exosomes and the anticancer activity.
活性氧物种已知与各种疾病有关，抗氧化成分有望从根本上预防疾病并有助于改善健康。然而，抗氧化剂在被肠道吸收之前很容易被酶降解，因此需要一种能够防止它们在体内降解的运输方式。外泌体在细胞间的通讯中起着重要作用，已作为 miRNA 和 DNA 的新型运输载体引起关注，但在食品研究中尚未得到充分利用。最近，从牛乳中提取的外泌体开始被广泛用作一种具有成本效益的运输载体，不仅在临床医学中，而且在功能性食品材料中也得到了广泛应用。为了将外泌体开发为功能性食品的载体，有必要进行系统的研究，以阐明封装物质的引入效率和性质。在本研究中，我们应用电穿孔和孵育将抗氧化剂封装到外泌体中，并研究了封装到外泌体中的效率和抗癌活性。
