Neves Ana Rute, Nunes Cláudia, Reis Salette
UCIBIO, REQUIMTE, Department of Chemical Sciences, Faculty of Pharmacy, University of Porto , Rua de Jorge Viterbo Ferreira, 228, 4050-313 Porto, Portugal.
J Phys Chem B. 2015 Sep 3;119(35):11664-72. doi: 10.1021/acs.jpcb.5b05419. Epub 2015 Aug 18.
Resveratrol has been widely studied because of its pleiotropic effects in cancer therapy, neuroprotection, and cardioprotection. It is believed that the interaction of resveratrol with biological membranes may play a key role in its therapeutic activity. The capacity of resveratrol to partition into lipid bilayers, its possible location within the membrane, and the influence of this compound on the membrane fluidity were investigated using membrane mimetic systems composed of egg l-α-phosphatidylcholine (EPC), cholesterol (CHOL), and sphingomyelin (SM). The results showed that resveratrol has greater affinity for the EPC bilayers than for EPC:CHOL [4:1] and EPC:CHOL:SM [1:1:1] membrane models. The increased difficulty in penetrating tight packed membranes is also demonstrated by fluorescence quenching of probes and by fluorescence anisotropy measurements. Resveratrol may be involved in the regulation of cell membrane fluidity, thereby contributing for cell homeostasis.
白藜芦醇因其在癌症治疗、神经保护和心脏保护方面的多效性作用而受到广泛研究。人们认为白藜芦醇与生物膜的相互作用可能在其治疗活性中起关键作用。使用由鸡蛋l-α-磷脂酰胆碱（EPC）、胆固醇（CHOL）和鞘磷脂（SM）组成的膜模拟系统，研究了白藜芦醇分配到脂质双层中的能力、其在膜内的可能位置以及该化合物对膜流动性的影响。结果表明，白藜芦醇对EPC双层的亲和力比对EPC:CHOL [4:1]和EPC:CHOL:SM [1:1:1]膜模型的亲和力更大。探针的荧光猝灭和荧光各向异性测量也证明了穿透紧密堆积膜的难度增加。白藜芦醇可能参与细胞膜流动性的调节，从而有助于细胞稳态。
