Chen Shuisen, Luo Ying, Ding Guangda, Xu Fangsen
National Key Laboratory of Crop Genetic Improvement, and Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China; Key Laboratory of Agricultural Biotechnology of Liaoning Province, College of Biological Science and Technology, Shenyang Agricultural University, Shenyang 110866, China.
National Key Laboratory of Crop Genetic Improvement, and Microelement Research Center, Huazhong Agricultural University, Wuhan 430070, China.
J Proteomics. 2016 Feb 5;133:144-152. doi: 10.1016/j.jprot.2015.12.020. Epub 2015 Dec 30.
Phosphorus (P) deficiency is a primary constraint for plant growth in terrestrial ecosystems. To better understand the genotypic differences in the adaptation mechanism of Brassica napus to P deficiency, we purified the plasma membrane (PM) from the roots of two genotypes: P-efficient "Eyou Changjia" and P-inefficient "B104-2". Combining label-free quantitative proteomics with the MaxQuant approach, a total of 71 proteins that significantly changed in abundances were identified in the two genotypes in response to P-free starvation, including 31 in "Eyou Changjia" and 40 in "B104-2". Based on comparative genomics study, 28 proteins were mapped to the confidence intervals of quantitative trait loci (QTLs) for P efficiency related traits. Seven decreased proteins with transporter activity were found to be located in the PM by subcellular localization analyses. These proteins involved in intracellular protein transport and ATP hydrolysis coupled proton transport were mapped to the QTL for P content and dry weight. Compared with "B104-2", more decreased proteins referring to transporter activity were found in "Eyou Changjia", showing that substance exchange was decreased in response to short-term P-free starvation. Together with the finding, more decreased proteins functioning in signal transduction and protein synthesis/degradation suggested that "Eyou Changjia" could slow the progression of growth and save more P in response to short-term P-free starvation.
P deficiency seriously limits the production and quality of B. napus. Roots absorb water and nutrients and anchor the plant in the soil. Therefore, to study root PM proteome under P stress would be helpful to understand the adaptation mechanism for P deficiency. However, PM proteome analysis in B. napus has been seldom reported due to the high hydrophobicity and low abundance of PM. Thus, we herein investigated the PM proteome alteration of roots in two B. napus genotypes, with different P deficient tolerances, in response to P-free starvation. The present study offers new insights and novel information for better understanding the adaptative response to P deficiency in B. napus.
磷（P）缺乏是陆地生态系统中植物生长的主要限制因素。为了更好地了解甘蓝型油菜对磷缺乏适应机制的基因型差异，我们从两种基因型的根中纯化了质膜（PM）：磷高效的“油优长荚”和磷低效的“B104 - 2”。结合无标记定量蛋白质组学和MaxQuant方法，在两种基因型中鉴定出总共71种丰度显著变化的蛋白质，这些蛋白质是对无磷饥饿的响应，其中“油优长荚”中有31种，“B104 - 2”中有40种。基于比较基因组学研究，28种蛋白质被定位到与磷效率相关性状的数量性状位点（QTL）的置信区间。通过亚细胞定位分析发现，7种具有转运活性的减少的蛋白质位于质膜中。这些参与细胞内蛋白质运输和ATP水解偶联质子运输的蛋白质被定位到磷含量和干重的QTL。与“B104 - 2”相比，“油优长荚”中发现更多涉及转运活性的减少的蛋白质，表明在短期无磷饥饿响应中物质交换减少。连同这一发现，更多在信号转导和蛋白质合成/降解中起作用的减少的蛋白质表明，“油优长荚”在短期无磷饥饿响应中可以减缓生长进程并节省更多的磷。
磷缺乏严重限制了甘蓝型油菜的产量和品质。根吸收水分和养分并将植物固定在土壤中。因此，研究磷胁迫下的根质膜蛋白质组将有助于理解对磷缺乏的适应机制。然而，由于质膜的高疏水性和低丰度，甘蓝型油菜中质膜蛋白质组分析很少被报道。因此，我们在此研究了两种对磷缺乏耐受性不同的甘蓝型油菜基因型的根质膜蛋白质组对无磷饥饿的响应变化。本研究为更好地理解甘蓝型油菜对磷缺乏的适应性反应提供了新的见解和新颖信息。
