Root Biology Center, State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Life Science and Technology School, Lingnan Normal University, Zhanjiang 524048, China.
Cells. 2022 Feb 14;11(4):651. doi: 10.3390/cells11040651.
Phosphorus (P) is an essential nutrient for plant growth. In recent decades, the application of phosphate (Pi) fertilizers has contributed to significant increases in crop yields all over the world. However, low efficiency of P utilization in crops leads to intensive application of Pi fertilizers, which consequently stimulates environmental pollution and exhaustion of P mineral resources. Therefore, in order to strengthen the sustainable development of agriculture, understandings of molecular mechanisms underlying P efficiency in plants are required to develop cultivars with high P utilization efficiency. Recently, a plant Pi-signaling network was established through forward and reverse genetic analysis, with the aid of the application of genomics, transcriptomics, proteomics, metabolomics, and ionomics. Among these, proteomics provides a powerful tool to investigate mechanisms underlying plant responses to Pi availability at the protein level. In this review, we summarize the recent progress of proteomic analysis in the identification of differential proteins that play roles in Pi acquisition, translocation, assimilation, and reutilization in plants. These findings could provide insights into molecular mechanisms underlying Pi acquisition and utilization efficiency, and offer new strategies in genetically engineering cultivars with high P utilization efficiency.
磷（P）是植物生长的必需养分。近几十年来，磷酸盐（Pi）肥料的应用使全球作物产量显著增加。然而，作物中 P 的利用效率低导致 Pi 肥料的大量施用，进而刺激了环境污染和 P 矿资源的枯竭。因此，为了加强农业的可持续发展，需要了解植物 P 效率的分子机制，以开发具有高 P 利用效率的品种。最近，通过正向和反向遗传学分析，并借助基因组学、转录组学、蛋白质组学、代谢组学和离子组学的应用，建立了一个植物 Pi 信号网络。在这些方法中，蛋白质组学为研究植物对 Pi 供应的响应机制提供了一个强大的工具，可在蛋白质水平上进行研究。在这篇综述中，我们总结了蛋白质组学分析在鉴定参与植物 Pi 吸收、转运、同化和再利用的差异蛋白方面的最新进展。这些发现可以深入了解 Pi 吸收和利用效率的分子机制，并为具有高 P 利用效率的品种的遗传工程提供新的策略。
