Salomão Priscila Maria Aranda, Comar Lívia Picchi, Buzalaf Marília Afonso Rabelo, Magalhães Ana Carolina
Department of Biological Sciences, Bauru School of Dentistry - University of São Paulo, Al. Dr. Octávio Pinheiro Brisolla 9-75, Bauru, SP, 17012-901, Brazil.
BMC Oral Health. 2016 Jan 8;16:2. doi: 10.1186/s12903-016-0160-9.
Artificial lesions produced by different protocols might directly influence the response to different remineralising treatments. This study compared the response of different artificial caries-like enamel lesions to home-care and professional fluoride based-remineralising treatments in situ.
The tested demineralising protocols were methylcellulose- MC gel, polyacrylic acid - PA gel, tetraethyl methylene diphosphanate - TEMDP solution, and acetate- Buffer solution. The lesions were remineralised using an in situ model, following a crossover and double blind design. Twelve subjects wore intra-oral appliances during 3 phases (3 d each): control (C) (saliva); home-care F(-) treatment (FD) (1,100 ppm F(-) dentifrice, 2x1 min/day); and professional (FVD) (22,600 ppm F(-) varnish) plus FD. The de-remineralisation was measured by transverse microradiography-TMR and hardness (surface hardness/cross-sectional hardness, SH/CSH, respectively).
For SH, lesions produced by PA gel were the only one showing significant differences among the remineralising treatments (C x FD x FVD); while the TEMDP lesion were not responsive to any fluoride treatment (for both SH/CSH). For TMR, there were no differences among the remineralising treatments, regardless of the type of lesion. Generally, the most responsive lesions to fluoride were the less demineralised lesions (considering hardness: PA gel and Buffer).
The type of lesion has influence on the surface remineralisation degree induced by home-care and professional fluoride treatments using this in situ model.
不同方案产生的人工损伤可能直接影响对不同再矿化治疗的反应。本研究在原位比较了不同人工龋样釉质损伤对家庭护理和专业氟化物再矿化治疗的反应。
测试的脱矿方案为甲基纤维素 - MC凝胶、聚丙烯酸 - PA凝胶、四乙基甲基二膦酸酯 - TEMDP溶液和醋酸盐缓冲溶液。采用交叉双盲设计,使用原位模型对损伤进行再矿化。12名受试者在3个阶段(每个阶段3天)佩戴口腔内装置:对照(C)(唾液);家庭护理F(-)治疗(FD)(含1100 ppm F(-)的牙膏,每天2次,每次1分钟);专业治疗(FVD)(含22600 ppm F(-)的氟漆)加FD。通过横向显微放射照相术 - TMR和硬度(分别为表面硬度/横截面硬度,SH/CSH)测量脱矿情况。
对于SH,PA凝胶产生的损伤是唯一在再矿化治疗(C×FD×FVD)之间显示出显著差异的损伤;而TEMDP损伤对任何氟化物治疗均无反应(对于SH/CSH两者)。对于TMR,无论损伤类型如何,再矿化治疗之间均无差异。一般来说,对氟化物反应最敏感的损伤是脱矿程度较低的损伤(考虑硬度:PA凝胶和缓冲液)。
使用该原位模型,损伤类型会影响家庭护理和专业氟化物治疗诱导的表面再矿化程度。