牛奶中氟化物的摄入频率和含量对釉质和牙本质人工龋再矿化的影响：体外/原位研究

Frequency of intake and amount of fluoride in milk for remineralisation of artificial caries on enamel and dentine: Ex vivo/in situ study.

作者信息

Cassiano Luiza, Pessan Juliano, Comar Livia, Levy Flavia, Cardoso Cristiane, Dionisio Aline, Manarelli Michele, Grizzo Larissa, Magalhães Ana Carolina, Buzalaf Marília

机构信息

Department of Biological Sciences, Bauru School of Dentistry, University of São Paulo, Bauru, SP, Al. Octávio Pinheiro Brisolla, 9-75, Bauru, SP, 17012-901, Brazil.

Araçatuba Dental School, UNESP - Univ Estadual Paulista, Araçatuba, SP, R. José Bonifácio, 1193, Araçatuba, SP, 16015-050, Brazil.

出版信息

Arch Oral Biol. 2017 Jan;73:136-141. doi: 10.1016/j.archoralbio.2016.10.005. Epub 2016 Oct 13.

DOI:10.1016/j.archoralbio.2016.10.005

PMID:27764682

Abstract

OBJECTIVES

This study analysed the effect of frequency of intake and amount of fluoride in milk on the remineralisation of artificial enamel and dentine caries lesions ex vivo/in situ.

MATERIALS AND METHODS

Pre-demineralised bovine enamel and dentine slabs were randomly allocated into 5 groups and fixed in removable appliances used by subjects for 7days in each phase. Each treatment comprised milk containing 2.5ppm fluoride daily (T1), or every other day (T2), 5.0ppm F daily (T3), or every other day (T4) or no treatment (T5).

RESULTS

Enamel alterations were quantified by surface hardness recovery (%SHR) and transversal microradiography (TMR), and in dentine by TMR only. Data were analysed by ANOVA and Tukey's test (p<0.05). For enamel, the highest %SHR was found for T1 and T3 compared to control, without significant differences between them. All groups showed positive values of ΔΔZ - T1 (247.3±198.5); T2 (110.9±303.2); T3 (226.0±299.2); T5 (5.0±288.0), except T4 (-274.5±407.3). For dentine, the only group that presented remineralisation was T2 (350.0±657.5).

CONCLUSIONS

Fluoridated milk daily seems to have higher remineralising effect on enamel than its use every other day. Dentine, does not seem to benefit from daily use of fluoridated milk.

摘要

目的

本研究分析了牛奶中氟的摄入频率和含量对人工釉质和牙本质龋损离体/原位再矿化的影响。

材料与方法

将预先脱矿的牛釉质和牙本质块随机分为5组，并固定在受试者使用的可摘矫治器中，每个阶段使用7天。每种处理包括每天含2.5ppm氟的牛奶（T1），或隔天含2.5ppm氟的牛奶（T2），每天含5.0ppm氟的牛奶（T3），或隔天含5.0ppm氟的牛奶（T4），或不进行处理（T5）。

结果

通过表面硬度恢复率（%SHR）和横向显微放射照相术（TMR）对釉质变化进行定量分析，对牙本质仅通过TMR进行分析。数据采用方差分析和Tukey检验（p<0.05）。对于釉质，与对照组相比，T1和T3组的%SHR最高，且两组之间无显著差异。所有组的ΔΔZ值均为正值 - T1（247.3±198.5）；T2（110.9±303.2）；T3（226.0±299.2）；T5（5.0±288.0），除了T4（-274.5±407.3）。对于牙本质，唯一呈现再矿化的组是T2（350.0±657.5）。