Bruggeman Quentin, Mazubert Christelle, Prunier Florence, Lugan Raphaël, Chan Kai Xun, Phua Su Yin, Pogson Barry James, Krieger-Liszkay Anja, Delarue Marianne, Benhamed Moussa, Bergounioux Catherine, Raynaud Cécile
Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.).
Institute of Plant Sciences Paris-Saclay (IPS2), UMR 9213/UMR1403, CNRS, INRA, Université Paris-Sud, Université d'Evry, Université Paris-Diderot, Sorbonne Paris-Cité, Bâtiment 630, 91405 Orsay, France (Q.B., C.M., F.P., M.D., M.B., C.B., C.R.);Institut de Biologie Moléculaire des Plantes, Unité Propre de Recherche 2357 CNRS, Université de Strasbourg, 12 rue du Général Zimmer, 67084 Strasbourg cedex, France (R.L.);Australian Research Council Centre of Excellence in Plant Energy Biology, Research School of Biology, Australian National University, Acton, Australian Capital Territory 2601, Australia (K.X.C., S.Y.P., B.J.P.);Institute for Integrative Biology of the Cell (I2BC), Commissariat à l'Energie Atomique et aux Energies Alternatives Saclay, Centre National de la Recherche Scientifique, Université Paris-Sud, F-91191 Gif-sur-Yvette cedex, France (A.K.-L.); and Division of Biological and Environmental Sciences and Engineering and Center for Desert Agriculture, King Abdullah University of Science and Technology, Thuwal, Kingdom of Saudi Arabia (M.B.)
Plant Physiol. 2016 Mar;170(3):1745-56. doi: 10.1104/pp.15.01872. Epub 2016 Jan 8.
Programmed cell death (PCD) is a crucial process both for plant development and responses to biotic and abiotic stress. There is accumulating evidence that chloroplasts may play a central role during plant PCD as for mitochondria in animal cells, but it is still unclear whether they participate in PCD onset, execution, or both. To tackle this question, we have analyzed the contribution of chloroplast function to the cell death phenotype of the myoinositol phosphate synthase1 (mips1) mutant that forms spontaneous lesions in a light-dependent manner. We show that photosynthetically active chloroplasts are required for PCD to occur in mips1, but this process is independent of the redox state of the chloroplast. Systematic genetic analyses with retrograde signaling mutants reveal that 3'-phosphoadenosine 5'-phosphate, a chloroplast retrograde signal that modulates nuclear gene expression in response to stress, can inhibit cell death and compromises plant innate immunity via inhibition of the RNA-processing 5'-3' exoribonucleases. Our results provide evidence for the role of chloroplast-derived signal and RNA metabolism in the control of cell death and biotic stress response.
程序性细胞死亡（PCD）对于植物发育以及对生物和非生物胁迫的响应而言都是一个关键过程。越来越多的证据表明，叶绿体在植物PCD过程中可能像动物细胞中的线粒体一样发挥核心作用，但尚不清楚它们是参与PCD的起始、执行，还是两者都参与。为了解决这个问题，我们分析了叶绿体功能对肌醇磷酸合酶1（mips1）突变体细胞死亡表型的贡献，该突变体以光依赖的方式形成自发损伤。我们发现，光合活性叶绿体是mips1中发生PCD所必需的，但这个过程与叶绿体的氧化还原状态无关。对逆行信号突变体进行的系统遗传分析表明，3'-磷酸腺苷5'-磷酸是一种叶绿体逆行信号，可响应胁迫调节核基因表达，它能够抑制细胞死亡，并通过抑制RNA加工5'-3'外切核糖核酸酶损害植物的先天免疫。我们的结果为叶绿体衍生信号和RNA代谢在控制细胞死亡和生物胁迫响应中的作用提供了证据。
