Laloglu Esra, Aksoy Hulya, Aksoy Yılmaz, Ozkaya Fatih, Akcay Fatih
1 Department of Medical Biochemistry, Medical School of Ataturk University, Erzurum, Turkey.
2 Department of Urology, Medical School of Ataturk University, Erzurum, Turkey.
Ann Clin Biochem. 2016 Nov;53(6):647-653. doi: 10.1177/0004563216629169. Epub 2016 Sep 28.
Background Endocan (endothelial cell-specific molecule-1) is a proteoglycan and plays an important role in angiogenesis and inflammation. The aim of this study was to evaluate of serum and urinary concentrations of endothelial cell-specific molecule-1 in bladder cancer. Methods The study included 50 bladder cancer patients, 50 with urinary tract infection and 51 healthy volunteers. Serum and urinary endothelial cell-specific molecule-1 concentrations were measured with enzyme linked immunosorbent assay. Results In bladder cancer group, serum and urinary endothelial cell-specific molecule-1 concentrations were significantly higher than in the healthy subjects ( P = 0.003 and P < 0.0001). Urinary endothelial cell-specific molecule-1 concentrations in cases with urinary tract infection were higher than in healthy volunteers ( P = 0.002). There were no significant differences between bladder cancer and urinary tract infection groups in terms of serum and urinary endothelial cell-specific molecule-1 concentrations. Urinary endothelial cell-specific molecule-1 concentrations were higher than those of corresponding serum endothelial cell-specific molecule-1 concentrations ( P < 0.0001 for bladder cancer and urinary tract infection groups, P = 0.002 for healthy subjects). In bladder cancer group, there was a positive correlation between serum endothelial cell-specific molecule-1 and urinary endothelial cell-specific molecule-1 concentrations ( r = 0.32, P = 0.002). For serum endothelial cell-specific molecule-1, sensitivity and specificity were 50%, and 77%, and for urinary endothelial cell-specific molecule-1, 62%, and 71%, respectively. Conclusion Serum and urinary endothelial cell-specific molecule-1 concentrations increase in bladder cancer. This parameter also increases in serum and urine of cases with urinary tract infection. That urinary endothelial cell-specific molecule-1 values were higher than serum endothelial cell-specific molecule-1 values in all groups may be attributed to direct exfoliation of epithelial cells in bladder to urine.
背景 内皮糖蛋白（内皮细胞特异性分子-1）是一种蛋白聚糖，在血管生成和炎症中起重要作用。本研究旨在评估膀胱癌患者血清和尿液中内皮细胞特异性分子-1的浓度。方法 本研究纳入50例膀胱癌患者、50例尿路感染患者和51名健康志愿者。采用酶联免疫吸附测定法检测血清和尿液中内皮细胞特异性分子-1的浓度。结果 膀胱癌组血清和尿液中内皮细胞特异性分子-1的浓度显著高于健康受试者（P = 0.003和P < 0.0001）。尿路感染患者尿液中内皮细胞特异性分子-1的浓度高于健康志愿者（P = 0.002）。膀胱癌组和尿路感染组血清和尿液中内皮细胞特异性分子-1的浓度无显著差异。尿液中内皮细胞特异性分子-1的浓度高于相应血清中内皮细胞特异性分子-1的浓度（膀胱癌组和尿路感染组P < 0.0001，健康受试者P = 0.002）。在膀胱癌组中，血清内皮细胞特异性分子-1与尿液内皮细胞特异性分子-1的浓度呈正相关（r = 0.32，P = 0.002）。血清内皮细胞特异性分子-1的敏感性和特异性分别为50%和77%，尿液内皮细胞特异性分子-1的敏感性和特异性分别为62%和71%。结论 膀胱癌患者血清和尿液中内皮细胞特异性分子-1的浓度升高。该参数在尿路感染患者的血清和尿液中也升高。所有组中尿液内皮细胞特异性分子-1值均高于血清内皮细胞特异性分子-1值，这可能归因于膀胱上皮细胞直接脱落至尿液中。
