Faburay Bonto, Gaudreault Natasha N, Liu Qinfang, Davis A Sally, Shivanna Vinay, Sunwoo Sun Young, Lang Yuekun, Morozov Igor, Ruder Mark, Drolet Barbara, Scott McVey D, Ma Wenjun, Wilson William, Richt Juergen A
Department of Diagnostic Medicine/Pathobiology, College of Veterinary Medicine, Kansas State University, Manhattan, Kansas, Unites States.
United States Department of Agriculture, Agricultural Research Service, Arthropod Borne Animal Disease Research Unit, Manhattan, Kansas, United States.
Virology. 2016 Feb;489:128-40. doi: 10.1016/j.virol.2015.12.003. Epub 2015 Dec 31.
Rift Valley fever (RVF) is a zoonotic disease that causes severe epizootics in ruminants, characterized by mass abortion and high mortality rates in younger animals. The development of a reliable challenge model is an important prerequisite for evaluation of existing and novel vaccines. A study aimed at comparing the pathogenesis of RVF virus infection in US sheep using two genetically different wild type strains of the virus (SA01-1322 and Kenya-128B-15) was performed. A group of sheep was inoculated with both strains and all infected sheep manifested early-onset viremia accompanied by a transient increase in temperatures. The Kenya-128B-15 strain manifested higher virulence compared to SA01-1322 by inducing more severe liver damage, and longer and higher viremia. Genome sequence analysis revealed sequence variations between the two isolates, which potentially could account for the observed phenotypic differences. We conclude that Kenya-128B-15 sheep infection represents a good and virulent challenge model for RVF.
裂谷热（RVF）是一种人畜共患疾病，可在反刍动物中引发严重的动物流行病，其特征为幼龄动物大量流产和高死亡率。建立可靠的攻毒模型是评估现有疫苗和新型疫苗的重要前提。开展了一项研究，旨在比较使用两种基因不同的野生型裂谷热病毒株（SA01 - 1322和肯尼亚 - 128B - 15）对美国绵羊进行裂谷热病毒感染的发病机制。一组绵羊接种了这两种毒株，所有受感染的绵羊均出现早期病毒血症，并伴有体温短暂升高。与SA01 - 1322相比，肯尼亚 - 128B - 15毒株诱导了更严重的肝损伤、更长时间和更高水平的病毒血症，表现出更高的毒力。基因组序列分析揭示了这两种分离株之间的序列差异，这可能是观察到的表型差异的原因。我们得出结论，肯尼亚 - 128B - 15毒株感染绵羊是裂谷热一种良好且具有毒力的攻毒模型。
