Du Wen, Prochazka Jan, Prochazkova Michaela, Klein Ophir D
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu, Sichuan, 610041, China; Department of Orofacial Sciences and Program in Craniofacial Biology, University of California San Francisco, San Francisco, CA 94143, USA.
Department of Orofacial Sciences and Program in Craniofacial Biology, University of California San Francisco, San Francisco, CA 94143, USA; Laboratory of Transgenic Models of Diseases, Institute of Molecular Genetics of the ASCR, v.v.i., Prague, Czech Republic.
Gene Expr Patterns. 2016 Mar;20(2):81-7. doi: 10.1016/j.gep.2015.12.003. Epub 2015 Dec 31.
The fibroblast growth factors (FGFs) constitute one of the largest growth factor families, and several ligands and receptors in this family are known to play critical roles during tongue development. In order to provide a comprehensive foundation for research into the role of FGFs during the process of tongue formation, we measured the transcript levels by quantitative PCR and mapped the expression patterns by in situ hybridization of all 22 Fgfs during mouse tongue development between embryonic days (E) 11.5 and E14.5. During this period, Fgf5, Fgf6, Fgf7, Fgf9, Fgf10, Fgf13, Fgf15, Fgf16 and Fgf18 could all be detected with various intensities in the mesenchyme, whereas Fgf1 and Fgf2 were expressed in both the epithelium and the mesenchyme. Our results indicate that FGF signaling regulates tongue development at multiple stages.
成纤维细胞生长因子（FGFs）构成了最大的生长因子家族之一，已知该家族中的几种配体和受体在舌发育过程中发挥关键作用。为了为研究FGFs在舌形成过程中的作用提供全面的基础，我们通过定量PCR测量了转录水平，并通过原位杂交绘制了在胚胎期（E）11.5至E14.5之间小鼠舌发育过程中所有22种Fgfs的表达模式。在此期间，Fgf5、Fgf6、Fgf7、Fgf9、Fgf10、Fgf13、Fgf15、Fgf16和Fgf18均可在间充质中以不同强度检测到，而Fgf1和Fgf2在上皮和间充质中均有表达。我们的结果表明，FGF信号在多个阶段调节舌发育。
