Departments of Biochemistry and Biomedical Sciences and Chemistry & Chemical Biology, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4K1, Canada.
Biointerfaces Institute, McMaster University, 1280 Main Street West, Hamilton, ON, L8S 4L8, Canada.
Angew Chem Int Ed Engl. 2016 Feb 18;55(8):2709-13. doi: 10.1002/anie.201509389. Epub 2016 Jan 8.
We report on a paper device capable of carrying out target-induced rolling circle amplification (RCA) to produce massive DNA amplicons that can be easily visualized. Interestingly, we observed that RCA was more proficient on paper than in solution, which we attribute to a significantly higher localized concentration of immobilized DNA. Furthermore, we have successfully engineered a fully functional paper device for sensitive DNA or microRNA detection via printing of all RCA-enabling molecules within a polymeric sugar film formed from pullulan, which was integrated with the paper device. This encapsulation not only stabilizes the entrapped reagents at room temperature but also enables colorimetric bioassays with minimal steps.
我们报告了一种纸基器件，它能够进行目标诱导的滚环扩增（RCA）以产生大量可轻易可视化的 DNA 扩增子。有趣的是，我们观察到，RCA 在纸基器件上比在溶液中更为高效，我们将其归因于固定化 DNA 的局部浓度显著提高。此外，我们通过在由普鲁兰多糖形成的聚合物糖膜内打印所有实现 RCA 的分子，成功地设计了一种完全功能化的纸基器件，用于通过 DNA 或 microRNA 的灵敏检测，该糖膜与纸基器件集成在一起。这种封装不仅能在室温下稳定包埋的试剂，还能通过最少的步骤进行比色生物测定。
