Bogaczewicz Jaroslaw, Malinowska Karolina, Sysa-Jedrzejowska Anna, Wozniacka Anna
Department of Dermatology and Venereology, Medical University of Lodz, Lodz, Poland.
Department of Social Sciences, University of Social Sciences, Lodz, Poland.
Int J Dermatol. 2016 Jul;55(7):e380-5. doi: 10.1111/ijd.13213. Epub 2016 Jan 8.
Effectiveness of ultraviolet (UV)A1 in flares of atopic dermatitis (AD) is thought to influence the expression of cytokines involved in its pathogenesis. The aim of the study was to investigate whether mRNA expression of human β defensin-1 (hβD-1) correlates with that of interleukin (IL)-4, IL-10, and IL-31 in skin lesions in AD before and after UVA1 phototherapy, to determine whether UVA1 decreases the expression of the aforementioned mediators, and to confirm whether changes in mRNA expression correspond with the clinical efficacy of UVA1.
Twenty-five patients with AD underwent medium-dose UVA1 phototherapy. Before and after UVA1, biopsies from acute skin lesions were studied using reverse transcription and real-time polymerase chain reaction.
Levels of mRNA hβD-1 correlated with those of IL-10 and IL-31, levels of IL-4 mRNA correlated with those of IL-10 and IL-31, and IL-10 expression correlated with that of IL-31, both before and after UVA1. Phototherapy with UVA1 improved SCORing of Atopic Dermatitis (SCORAD) values, decreased pruritus, and increased expression of IL-4. After UVA1, no difference was found in the mRNA expression of other molecules. The SCORAD index did not correlate with the expression of any examined mRNA either before or after UVA1.
hβD-1, IL-4, IL-10, and IL-31 are expressed in acute skin lesions in AD, and their levels correlate with each other. UVA1 improves SCORAD and pruritus and increases the expression of IL-4 without direct effect on other molecules.
长波紫外线A1（UVA1）对特应性皮炎（AD）皮疹的疗效被认为会影响其发病机制中相关细胞因子的表达。本研究旨在探讨UVA1光疗前后AD皮肤损伤中人类β-防御素-1（hβD-1）的mRNA表达与白细胞介素（IL）-4、IL-10和IL-31的mRNA表达是否相关，确定UVA1是否会降低上述介质的表达，并确认mRNA表达的变化是否与UVA1的临床疗效相对应。
25例AD患者接受中剂量UVA1光疗。在UVA1治疗前后，采用逆转录和实时聚合酶链反应对急性皮肤损伤的活检组织进行研究。
UVA1治疗前后，hβD-1的mRNA水平与IL-10和IL-31的水平相关，IL-4的mRNA水平与IL-10和IL-31的水平相关，且IL-10的表达与IL-31的表达相关。UVA1光疗改善了特应性皮炎评分（SCORAD）值，减轻了瘙痒，并增加了IL-4的表达。UVA1治疗后，其他分子的mRNA表达未发现差异。UVA1治疗前后，SCORAD指数与任何检测的mRNA表达均无相关性。
hβD-1、IL-4、IL-10和IL-31在AD的急性皮肤损伤中表达，且它们的水平相互关联。UVA1改善了SCORAD和瘙痒症状，并增加了IL-4的表达，而对其他分子无直接影响。
