Bogaczewicz Jaroslaw, Malinowska Karolina, Sysa-Jedrzejowska Anna, Wozniacka Anna
Department of Dermatology and Venereology, Medical University of Lodz, Lodz, Poland.
Department of Social Sciences, University of Social Sciences, Lodz, Poland.
Int J Dermatol. 2016 Aug;55(8):856-63. doi: 10.1111/ijd.12992. Epub 2015 Oct 16.
The mechanisms responsible for the efficacy of ultraviolet A1 (UVA1) in the treatment of atopic dermatitis (AD) are not fully understood.
This study was designed to investigate mRNA expression of thymic stromal lymphopoietin (TSLP), thymus- and activation-regulated chemokine (TARC), interleukin-5 (IL-5), and IL-13 in AD before and after UVA1 therapy, to determine correlations among them, and to examine whether UVA1 influences their expression and whether it is associated with UVA1 efficacy.
Twenty-five patients with AD underwent medium-dose UVA1 phototherapy. Before and after UVA1, biopsies from acute skin lesions were studied using reverse transcription and real-time polymerase chain reaction.
Levels of mRNA TSLP correlated with those of TARC, IL-5, and IL-13, and levels of TARC correlated with those of IL-5 and IL-13, both before and after UVA1. Expression of IL-5 correlated with that of IL-13 only before UVA1. SCORAD (SCORing of Atopic Dermatitis) indices correlated with levels of TARC and IL-5 before irradiation. After UVA1, no mRNA level correlated with the SCORAD index. Phototherapy with UVA1 improved SCORAD values (P < 0.001) and increased expression of TARC (P < 0.05) but did not affect mRNA expression of TSLP, IL-5, or IL-13.
Expression levels of the mediators TSLP, TARC, IL-5, and IL-13 in AD are interrelated. Phototherapy with UVA1 improves SCORAD indices and increases expression of TARC but has no direct effects on the expression of other molecules. It is likely that UVA1 also interferes with or acts via intermediators on the link between IL-5 and IL-13.
紫外线A1（UVA1）治疗特应性皮炎（AD）的有效机制尚未完全明确。
本研究旨在调查UVA1治疗前后AD患者中胸腺基质淋巴细胞生成素（TSLP）、胸腺和活化调节趋化因子（TARC）、白细胞介素-5（IL-5）及IL-13的mRNA表达，确定它们之间的相关性，并研究UVA1是否影响其表达以及是否与UVA1的疗效相关。
25例AD患者接受中剂量UVA1光疗。在UVA1治疗前后，采用逆转录和实时聚合酶链反应对急性皮肤病变活检组织进行研究。
UVA1治疗前后，TSLP的mRNA水平均与TARC、IL-5及IL-13的水平相关，TARC的水平与IL-5及IL-13的水平相关。仅在UVA1治疗前，IL-5的表达与IL-13的表达相关。照射前，特应性皮炎评分（SCORAD）指数与TARC和IL-5的水平相关。UVA1治疗后，无mRNA水平与SCORAD指数相关。UVA1光疗改善了SCORAD值（P < 0.001），并增加了TARC的表达（P < 0.05），但未影响TSLP、IL-5或IL-13的mRNA表达。
AD中介质TSLP、TARC、IL-5和IL-13的表达水平相互关联。UVA1光疗可改善SCORAD指数并增加TARC的表达，但对其他分子的表达无直接影响。UVA1可能还通过中间介质干扰或作用于IL-5和IL-13之间的联系。
