Negi Anjana Kumari, Bhatnagar Archana, Agnihotri Navneet
Department of Biochemistry, Panjab University, Chandigarh, 160014, India.
Inflammopharmacology. 2016 Feb;24(1):11-22. doi: 10.1007/s10787-015-0259-7. Epub 2016 Jan 9.
Chronic inflammation has been directly linked to cancer progression. Therefore, current study was designed to understand the mechanism of action of chemo-preventive effect of celecoxib and fish oil on inflammatory mediators in experimental mammary carcinoma. Female Wistar rats were distributed into control and DMBA treated groups and further subdivided based on pretreatment with celecoxib and/or fish oil. Inflammation was measured by assessing expression of NF-κB, COX-2 and cytokines. The results indicated an elevation in expression of NF-κB, COX-2 and cytokines' levels (IFN-γ, IL-4 and IL-10) in DMBA group as compared to controls. On pretreatment with celecoxib and/or fish oil in DMBA treated animals, a significant reduction in expression of NF-κB, COX-2 and cytokines' levels was observed. The decrease was more pronounced with combinatorial regimen than either celecoxib or fish oil alone. To conclude, a combinatorial strategy of celecoxib and fish oil may generate an immune response against the tumor cell by altering cytokine repertoire and decrease the tendency of tumor cells to escape immune surveillance.
慢性炎症与癌症进展直接相关。因此，本研究旨在了解塞来昔布和鱼油对实验性乳腺癌炎症介质的化学预防作用机制。将雌性Wistar大鼠分为对照组和经二甲基苯并蒽（DMBA）处理的组，并根据塞来昔布和/或鱼油的预处理进一步细分。通过评估核因子κB（NF-κB）、环氧化酶-2（COX-2）和细胞因子的表达来测量炎症。结果表明，与对照组相比，DMBA组中NF-κB、COX-2的表达以及细胞因子水平（干扰素-γ（IFN-γ）、白细胞介素-4（IL-4）和白细胞介素-10（IL-10））升高。在经DMBA处理的动物中用塞来昔布和/或鱼油进行预处理后，观察到NF-κB、COX-2的表达以及细胞因子水平显著降低。联合用药方案的降低效果比单独使用塞来昔布或鱼油更明显。总之，塞来昔布和鱼油的联合策略可能通过改变细胞因子谱产生针对肿瘤细胞的免疫反应，并降低肿瘤细胞逃避免疫监视的倾向。
