Fasano Giovanna, Fontenelle Nicolas, Vannin Anne-Sophie, Biramane Jamila, Devreker Fabienne, Englert Yvon, Delbaere Anne
Research Laboratory on Human Reproduction, Faculty of Medicine, Université Libre de Bruxelles, Campus Erasme, Bruxelles, Belgium,
J Assist Reprod Genet. 2014 Feb;31(2):241-7. doi: 10.1007/s10815-013-0145-4. Epub 2013 Dec 8.
To compare two different vitrification methods to slow freezing method for cryopreservation of human cleavage stage embryos.
Prospective randomised trial.
University assisted reproduction centre.
PATIENT(S): 568 patients (mean age 33.4 ± 5.2) from April 2009 to April 2011.
1798 supernumerary good-quality cleavage stage embryos in 645 IVF cycles intended to be cryopreserved were randomly allocated to three groups: slow freezing, vitrification with the Irvine® method, vitrification with the Vitrolife® method.
MAIN OUTCOME MEASURE(S): Embryo survival and cleavage rates, implantation rate.
A total of 1055 embryos were warmed, 836 (79.2%) survived and 676 were finally transferred (64.1%). Post-warming embryos survival rate was significantly higher after vitrification (Irvine: 89.4%; Vitrolife: 87.6%) than after slow freezing (63.8%) (p < 0.001). No differences in survival rates were observed between the two vitrification methods, but a significant higher cleavage rate was observed using Irvine compared to Vitrolife method (p < 0.05). Implantation rate (IR) per embryo replaced and per embryo warmed were respectively 15.8% (41/259) and 12.4% (41/330) for Irvine, 17.0% (40/235) and 12.1% (40/330) for Vitrolife, 21.4% (39/182) and 9.9% (39/395) for slow-freezing (NS).
Both vitrification methods (Irvine and Vitrolife) are more efficient than slow freezing for cryopreservation of human cleavage stage embryos in terms of post-warming survival rate. No significant difference in the implantation rate was observed between the three cryopreservation methods.
比较两种不同的玻璃化冷冻方法与慢速冷冻法用于人类卵裂期胚胎冷冻保存的效果。
前瞻性随机试验。
大学辅助生殖中心。
2009年4月至2011年4月期间的568例患者（平均年龄33.4±5.2岁）。
在645个拟进行冷冻保存的体外受精周期中，将1798个优质的多余卵裂期胚胎随机分为三组：慢速冷冻组、使用Irvine®方法的玻璃化冷冻组、使用Vitrolife®方法的玻璃化冷冻组。
胚胎存活率、卵裂率、着床率。
共解冻1055个胚胎，836个（79.2%）存活，最终移植676个（64.1%）。玻璃化冷冻后（Irvine法：89.4%；Vitrolife法：87.6%）的解冻后胚胎存活率显著高于慢速冷冻后（63.8%）（p<0.001）。两种玻璃化冷冻方法的存活率无差异，但与Vitrolife法相比，Irvine法的卵裂率显著更高（p<0.05）。Irvine法每个移植胚胎和每个解冻胚胎的着床率分别为15.8%（41/259）和12.4%（41/330），Vitrolife法分别为17.0%（40/235）和12.1%（40/330），慢速冷冻法分别为21.4%（39/182）和9.9%（39/395）（无显著性差异）。
就解冻后存活率而言，两种玻璃化冷冻方法（Irvine法和Vitrolife法）用于人类卵裂期胚胎冷冻保存均比慢速冷冻更有效。三种冷冻保存方法的着床率无显著差异。
