普通麦瓶草毛状根培养物中三萜皂苷元的生产

Production of Triterpenoid Sapogenins in Hairy Root Cultures of Silene vulgaris.

作者信息

Kim Yeon Bok, Reed Darwin W, Covello Patrick S

出版信息

Nat Prod Commun. 2015 Nov;10(11):1919-22.

Abstract

Silene vulgaris (Moench) Garcke (Caryophyllaceae) is widely distributed in North America and contains bioactive oleanane-type saponins. In order to investigate in vitro production of triterpenoid saponins, hairy root cultures of S. vulgaris were established by infecting leaf explants with five strains of Agrobacterium rhizogenes (LBA9402, R1000, A4, 13333, and 15834). The A. rhizogenes strain LBA9402 had an infection of 100% frequency and induced the most hairy roots per plant. Methyl jasmonate (MeJA)-induced changes in triterpenoid saponins in S. vulgaris hairy roots were analyzed. Accumulation of segetalic acid and gypsogenic acid after MeJA treatment was 5-and 2-fold higher, respectively, than that of control root. We suggest that hairy root cultures of S. vulgaris could be an important alternative approach to the production of saponins.

摘要

蝇子草（Silene vulgaris (Moench) Garcke，石竹科）广泛分布于北美，含有具有生物活性的齐墩果烷型皂苷。为了研究三萜皂苷的体外生产，通过用五株发根农杆菌（LBA9402、R1000、A4、13333和15834）感染叶片外植体，建立了蝇子草的毛状根培养体系。发根农杆菌菌株LBA9402的感染频率为100%，且每株诱导产生的毛状根最多。分析了茉莉酸甲酯（MeJA）诱导的蝇子草毛状根中三萜皂苷的变化。MeJA处理后，隔离酸和石膏酸的积累分别比对照根高5倍和2倍。我们认为，蝇子草的毛状根培养可能是皂苷生产的一种重要替代方法。