Lee Sook-Young, Cho Soo-In, Park Min-Hee, Kim Yong-Kyung, Choi Jae-Eul, Park Sang-Un
Research Center for Proteineous Materials, Chosun University, Gwangju, Korea.
Prep Biochem Biotechnol. 2007;37(3):239-46. doi: 10.1080/10826060701386729.
Buckwheat (Fagopyrum esculentum Moench.) is a potentially important source of rutin, a natural flavonoid with antihyperglycemic, antihypertensive, and antioxidative properties. To examine in vitro production of rutin, we established a hairy root culture of buckwheat by infecting leaf explants with Agrobacterium rhizogenes R1000, and tested the growth conditions and rutin production rates of these cultures. Ten hairy root clones were established; their growth and rutin production rates ranged from 233 to 312 (mg dry wt per 30 mL flask, and 0.8 to 1.2 (mg/g dry wt), respectively. Clone H8, which had high growth and rutin production rates (312 mg dry wt per 30 mL flask and 1.2 mg/g dry wt, respectively), was selected for further experiments. H8 showed maximal growth and rutin content at 30 days in culture in MS medium. Of four tested culture media, half-strength MS medium was found to induce the highest levels of growth (378 mg dry wt per 30 mL flask) and rutin production (1.4 mg/g dry wt) by clone H8. In contrast, supplementation with auxins (0.1-1 mg/l IAA, IBA and NAA) increased the growth rate, but had no significant effect on rutin production by H8. Collectively, these findings indicate that hairy root cultures of buckwheat culture could be a valuable alternative approach for rutin production.
荞麦（苦荞麦）是芦丁的一个潜在重要来源，芦丁是一种具有降血糖、降血压和抗氧化特性的天然黄酮类化合物。为了研究芦丁的体外生产，我们通过用发根农杆菌R1000感染叶片外植体建立了荞麦毛状根培养物，并测试了这些培养物的生长条件和芦丁生产率。建立了10个毛状根克隆；它们的生长率和芦丁生产率分别为每30 mL烧瓶233至312（mg干重）和0.8至1.2（mg/g干重）。选择生长率和芦丁生产率高的克隆H8（分别为每30 mL烧瓶312 mg干重和1.2 mg/g干重）进行进一步实验。H8在MS培养基中培养30天时显示出最大生长和芦丁含量。在四种测试培养基中，发现1/2强度MS培养基诱导克隆H8的生长水平最高（每30 mL烧瓶378 mg干重）和芦丁产量最高（1.4 mg/g干重）。相比之下，添加生长素（0.1 - 1 mg/l IAA、IBA和NAA）提高了生长率，但对H8的芦丁产量没有显著影响。总的来说，这些发现表明荞麦毛状根培养物可能是芦丁生产的一种有价值的替代方法。
