Park Sang Eon, Lee Na Kyung, Lee Jeongmin, Hwang Jung Won, Choi Soo Jin, Hwang Hyeri, Hyung Brian, Chang Jong Wook, Na Duk L
aSamsung Advanced Institute for Health Science and Technology, Sungkyunkwan University bDepartment of Neurology, Samsung Medical Center, 50 Ilwon-dong, Gangnam-ku, Seoul 135-710, South Korea cNeuroscience Center, Samsung Medical Center, Seoul, Republic of Korea dStem Cell & Regenerative Medicine Center, Research Institute for Future Medicine, Samsung Medical Center, Seoul eBiomedical Research Institute, MEDIPOST Co. Ltd, Gyeonggi-do, Republic of Korea fKing's College London, London, UK gMcGill University, Montreal, Quebec, Canada.
Neuroreport. 2016 Mar 2;27(4):235-41. doi: 10.1097/WNR.0000000000000526.
The aim of this study was to track the migration of human umbilical cord blood-derived mesenchymal stem cells (hUCB-MSCs) administered through a single intravenous injection and to observe the consequential therapeutic effects in a transgenic Alzheimer's disease mouse model. Ten-month-old APP/PS1 mice received a total injection of 1×10 cells through the lateral tail vein and were killed 1, 4, and 7 days after administration. On the basis of immunohistochemical analysis, hUCB-MSCs were not detected in the brain at any of the time points. Instead, most of the injected mesenchymal stem cells were found to be distributed in the lung, heart, and liver. In terms of the molecular effects, statistically significant differences in the amyloid β protein, neprilysin, and SOX2 levels were not observed among the groups. On the basis of the results from this study, we suggest that single intravenously administered hUCB-MSCs are not delivered to the brain and also do not have a significant influence on Alzheimer's disease pathology.
本研究的目的是追踪单次静脉注射的人脐带血间充质干细胞（hUCB-MSCs）的迁移情况，并观察其在转基因阿尔茨海默病小鼠模型中的后续治疗效果。10月龄的APP/PS1小鼠通过侧尾静脉接受了总量为1×10⁶个细胞的注射，并在给药后1天、4天和7天处死。基于免疫组织化学分析，在任何时间点的大脑中均未检测到hUCB-MSCs。相反，发现大多数注射的间充质干细胞分布在肺、心脏和肝脏中。在分子效应方面，各实验组之间在淀粉样β蛋白、中性内肽酶和SOX2水平上未观察到统计学上的显著差异。基于本研究的结果，我们认为单次静脉注射的hUCB-MSCs不会进入大脑，并且对阿尔茨海默病的病理也没有显著影响。
