Wasiak Iga, Kulikowska Aleksandra, Janczewska Magdalena, Michalak Magdalena, Cymerman Iwona A, Nagalski Andrzej, Kallinger Peter, Szymanski Wladyslaw W, Ciach Tomasz
Warsaw University of Technology, Faculty of Chemical Engineering, Warynskiego 1, 00-645 Warsaw, Poland.
NanoVelos Sp. z o.o., Warynskiego 1 / 212, 00-645 Warsaw, Poland.
PLoS One. 2016 Jan 11;11(1):e0146237. doi: 10.1371/journal.pone.0146237. eCollection 2016.
Dextran is widely exploited in medical products and as a component of drug-delivering nanoparticles (NPs). Here, we tested whether dextran can serve as the main substrate of NPs and form a stable backbone. We tested dextrans with several molecular masses under several synthesis conditions to optimize NP stability. The analysis of the obtained nanoparticles showed that dextran NPs that were synthesized from 70 kDa dextran with a 5% degree of oxidation of the polysaccharide chain and 50% substitution with dodecylamine formed a NP backbone composed of modified dextran subunits, the mean diameter of which in an aqueous environment was around 100 nm. Dextran NPs could be stored in a dry state and reassembled in water. Moreover, we found that different chemical moieties (e.g., drugs such as doxorubicin) can be attached to the dextran NPs via a pH-dependent bond that allows release of the drug with lowering pH. We conclude that dextran NPs are a promising nano drug carrier.
葡聚糖在医疗产品中被广泛应用，并且作为药物递送纳米颗粒（NPs）的一种成分。在此，我们测试了葡聚糖是否可作为纳米颗粒的主要底物并形成稳定的骨架。我们在几种合成条件下测试了具有不同分子量的葡聚糖，以优化纳米颗粒的稳定性。对所得纳米颗粒的分析表明，由70 kDa葡聚糖合成的纳米颗粒，其多糖链氧化度为5%且十二烷基胺取代度为50%，形成了由修饰的葡聚糖亚基组成的纳米颗粒骨架，其在水性环境中的平均直径约为100 nm。葡聚糖纳米颗粒可以以干燥状态储存并在水中重新组装。此外，我们发现不同的化学基团（如阿霉素等药物）可以通过pH依赖性键连接到葡聚糖纳米颗粒上，这种键允许药物在pH降低时释放。我们得出结论，葡聚糖纳米颗粒是一种有前景的纳米药物载体。
