Taylor P M, Rose M L, Yacoub M H
Immunology Department, Harefield Hospital, Middlesex, England.
Transplantation. 1989 Sep;48(3):506-10. doi: 10.1097/00007890-198909000-00030.
Immunocytochemical techniques have been used to investigate the expression of common determinants of class I, common determinants of class II and DR, DP, and DQ antigens on frozen sections from twenty normal donor lungs, three lungs resected due to carcinoma and nine lungs removed from heart/lung recipients who were undergoing retransplant due to obliterative bronchiolitis. In all lungs, alveolar macrophages expressed common determinants of class I and class II, as well as DR, DP and DQ antigens. In normal lung, class I was expressed on all vascular endothelium, all tracheal epithelium and most, but not all, bronchiolar epithelium. Class II was always expressed on tracheal epithelium, but expression on bronchiolar epithelium and vascular endothelium was variable and sometimes absent. In lungs from transplant patients with severe obliterative bronchiolitis, vascular endothelium, in addition to expressing class I, now consistently expressed class II. Epithelium from trachea, bronchioles, and alveoli also consistently expressed class I and class II. To conclude, there is enhanced expression of class II antigens on endothelial and epithelial cells from lungs with obliterative bronchiolitis.
免疫细胞化学技术已用于研究来自20例正常供体肺、3例因癌症切除的肺以及9例因闭塞性细支气管炎而接受再次移植的心肺移植受者所切除肺的冰冻切片上I类共同决定簇、II类共同决定簇以及DR、DP和DQ抗原的表达情况。在所有肺中，肺泡巨噬细胞均表达I类和II类共同决定簇以及DR、DP和DQ抗原。在正常肺中，I类在所有血管内皮、所有气管上皮以及大部分（但并非全部）细支气管上皮中表达。II类始终在气管上皮中表达，但在细支气管上皮和血管内皮中的表达可变，有时缺失。在患有严重闭塞性细支气管炎的移植患者的肺中，血管内皮除了表达I类外，现在一致表达II类。气管、细支气管和肺泡的上皮也一致表达I类和II类。总之，闭塞性细支气管炎患者肺的内皮细胞和上皮细胞上II类抗原表达增强。
