Huang Wuren, Zhang Jie, Yang Bing, Beerntsen Brenda T, Song Hongsheng, Ling Erjun
Environment and Plant Protection Institute, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China.
Key Laboratory of Insect Developmental and Evolutionary Biology, Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China.
Sci Rep. 2016 Jan 12;6:19142. doi: 10.1038/srep19142.
Invertebrate animals have the capacity of repairing wounds in the skin and gut via different mechanisms. Gastrointestinal perforation, a hole in the human gastrointestinal system, is a serious condition, and surgery is necessary to repair the perforation to prevent an abdominal abscess or sepsis. Here we report the repair of gastrointestinal perforation made by a needle-puncture wound in the silkworm larval midgut. Following insect gut perforation, only a weak immune response was observed because the growth of Escherichia coli alone was partially inhibited by plasma collected at 6 h after needle puncture of the larval midgut. However, circulating hemocytes did aggregate over the needle-puncture wound to form a scab. While, cell division and apoptosis were not observed at the wound site, the needle puncture significantly enhanced DNA duplication in cells surrounding the wound, which was essential to repair the midgut perforation. Due to the repair capacity and limited immune response caused by needle puncture to the midgut, this approach was successfully used for the injection of small compounds (ethanol in this study) into the insect midgut. Consequently, this needle-puncture wounding of the insect gut can be developed for screening compounds for use as gut chemotherapeutics in the future.
无脊椎动物能够通过不同机制修复皮肤和肠道的伤口。胃肠穿孔，即人体胃肠系统中的一个洞，是一种严重的病症，需要进行手术来修复穿孔以防止腹腔脓肿或败血症。在此我们报告了家蚕幼虫中肠针刺伤口所致胃肠穿孔的修复情况。昆虫肠道穿孔后，仅观察到微弱的免疫反应，因为在幼虫中肠针刺后6小时收集的血浆仅部分抑制了大肠杆菌的生长。然而，循环血细胞确实在针刺伤口处聚集形成痂。虽然在伤口部位未观察到细胞分裂和凋亡，但针刺显著增强了伤口周围细胞中的DNA复制，这对于修复中肠穿孔至关重要。由于针刺中肠引起的修复能力和有限的免疫反应，这种方法成功用于将小化合物（本研究中的乙醇）注入昆虫中肠。因此，这种昆虫肠道的针刺伤口可在未来用于筛选用作肠道化疗药物的化合物。
