Janwan Penchom, Intapan Pewpan M, Yamasaki Hiroshi, Rodpai Rutchanee, Laummaunwai Porntip, Thanchomnang Tongjit, Sanpool Oranuch, Kobayashi Kaoru, Takayama Katsuyoshi, Kobayashi Yukuharu, Maleewong Wanchai
Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, 40002, Thailand.
Department of Medical Technology, School of Allied Health Sciences and Public Health, Walailak University, Nakhon Si Thammarat, 80161, Thailand.
Parasit Vectors. 2016 Jan 12;9:14. doi: 10.1186/s13071-016-1294-y.
Human gnathostomiasis is a serious tropical disease, which is often overlooked. There is an urgent need to improve tools to aid the potential diagnosis of the disease in endemic regions. To overcome this, we produced the immunochromatographic test (ICT) kit for a rapid and simple diagnosis of human gnathostomiasis.
The recombinant protein (named rGslic18) was applied to ICT kit as the antigen. The diagnostic value of ICT kit was evaluated using serum samples from parasitologically proven and clinically suspected gnathostomiasis patients, healthy volunteers and patients with other parasitic diseases. The ICT kit exhibited quite high sensitivity (93.75%) and specificity (97.01%).
The ICT kit is simple, convenient and easy to implement and expected to provide reliable diagnostic results for human gnathostomiasis. It also will be a promising diagnostic tool not only for large-scale epidemiological surveys in endemic or remote areas where diagnostic facilities are poor but also for a rapid clinical diagnosis in the bedside laboratory.
人体颚口线虫病是一种严重的热带疾病，常被忽视。迫切需要改进工具以辅助在流行地区对该疾病进行潜在诊断。为克服这一问题，我们生产了用于快速、简单诊断人体颚口线虫病的免疫层析检测（ICT）试剂盒。
重组蛋白（命名为rGslic18）作为抗原应用于ICT试剂盒。使用来自经寄生虫学证实和临床疑似颚口线虫病患者、健康志愿者以及其他寄生虫病患者的血清样本评估ICT试剂盒的诊断价值。该ICT试剂盒表现出相当高的灵敏度（93.75%）和特异性（97.01%）。
该ICT试剂盒简单、方便且易于实施，有望为人体颚口线虫病提供可靠的诊断结果。它不仅将成为在诊断设施匮乏的流行或偏远地区进行大规模流行病学调查的有前景的诊断工具，也将成为床边实验室快速临床诊断的工具。
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