Department of Parasitology, Faculty of Medicine, Khon Kaen University, 40002 Khon Kaen, Thailand - Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand.
Mekong Health Science Research Institute, Khon Kaen University, 40002 Khon Kaen, Thailand - Department of Medical Technology, School of Allied Health Sciences, Walailak University, 80161 Nakhon Si Thammarat, Thailand - Hematology and Transfusion Science Research Center, Walailak University, 80161 Nakhon Si Thammarat, Thailand.
Parasite. 2023;30:60. doi: 10.1051/parasite/2023063. Epub 2023 Dec 13.
Strongyloidiasis is a neglected tropical disease that can cause fatal complications due to hyperinfection and disseminated strongyloidiasis in immunocompromised patients. We used two Strongyloides stercoralis recombinant antigenic proteins, L3NieAg.01 (NIE) and IgG-immunoreactive antigen (SsIR), to develop the recombinant antigen-based immunochromatography test (ICT) kit. We constructed and compared kits using either the NIE (NIE ICT kit) or the SsIR (SsIR ICT kit) antigens and a kit using a mixture of both (NIE-SsIR ICT kit) for detection of anti-Strongyloides IgG antibody in human serum samples. Serum samples from normal healthy individuals (Group I, n = 40), proven strongyloidiasis patients (Group II, n = 100), and those with other parasitic infections (Group III, n = 154) were evaluated. Sensitivity and specificity were 81.0% and 84.0% for the NIE ICT kit, 89.0% and 83.5% for the SsIR ICT kit, and 95.0% and 90.2% for the NIE-SsIR ICT kit, respectively. The NIE-SsIR ICT kit provided the best diagnostic results; it can supplement stool examination for clinical diagnosis and can be used to screen for asymptomatic S. stercoralis infection in people at risk in endemic areas. The NIE-SsIR ICT kit can also be used in large-scale sero-epidemiological investigations in endemic areas without the need for additional facilities or ancillary supplies.
粪类圆线虫病是一种被忽视的热带病，由于免疫功能低下患者的重度感染和播散性粪类圆线虫病，可导致致命的并发症。我们使用两种粪类圆线虫重组抗原蛋白，L3NieAg.01（NIE）和 IgG-免疫反应性抗原（SsIR），开发了基于重组抗原的免疫层析检测试剂盒（ICT）。我们构建并比较了使用 NIE（NIE ICT 试剂盒）或 SsIR（SsIR ICT 试剂盒）抗原的试剂盒，以及使用两种抗原混合物（NIE-SsIR ICT 试剂盒）的试剂盒，用于检测人血清样本中的抗粪类圆线虫 IgG 抗体。评估了来自正常健康个体（I 组，n=40）、确诊粪类圆线虫病患者（II 组，n=100）和其他寄生虫感染患者（III 组，n=154）的血清样本。NIE ICT 试剂盒的灵敏度和特异性分别为 81.0%和 84.0%，SsIR ICT 试剂盒的灵敏度和特异性分别为 89.0%和 83.5%，NIE-SsIR ICT 试剂盒的灵敏度和特异性分别为 95.0%和 90.2%。NIE-SsIR ICT 试剂盒提供了最佳的诊断结果；它可以补充粪便检查用于临床诊断，并可用于筛查流行地区有风险人群的无症状粪类圆线虫感染。NIE-SsIR ICT 试剂盒也可用于流行地区的大规模血清流行病学调查，无需额外的设施或辅助用品。
