Clifford R D, Radford M, Howell J B, Holgate S T
Department of Medicine, University of Southampton.
Arch Dis Child. 1989 Aug;64(8):1126-32. doi: 10.1136/adc.64.8.1126.
A sample of 397 from 2503 children aged 7 and 11 years, who took part in an earlier questionnaire study of respiratory symptoms in Southampton, was chosen for methacholine bronchial provocation and allergen skin testing. Results were obtained from 330 (84%). A good level of repeatability was achieved for bronchial provocation testing giving a 95% confidence interval equivalent to 1.74 doubling concentrations. The main problem encountered with methacholine was its bitter taste in high concentrations. The proportion of children, in whom the dose of methacholine which produced a fall of 20% in the forced expiratory volume in one second (PD20 methacholine) was less than or equal to 6.4 mumol, fell from 29.1% at 7 years to 16.5% at 11 years. The prevalence of atopy increased from 26% at 7 years to 31.6% at 11 years. A highly significant association was found between bronchial responsiveness to methacholine and atopy, which was independent of symptoms or age group.
从2503名7至11岁儿童中抽取了397名样本，这些儿童参与了南安普敦一项早期的呼吸道症状问卷调查研究，被选来进行乙酰甲胆碱支气管激发试验和变应原皮肤试验。330名（84%）获得了结果。支气管激发试验的重复性良好，其95%置信区间相当于1.74倍浓度。乙酰甲胆碱遇到的主要问题是其高浓度时的苦味。一秒用力呼气量下降20%时乙酰甲胆碱剂量（PD20乙酰甲胆碱）小于或等于6.4微摩尔的儿童比例，从7岁时的29.1%降至11岁时的16.5%。特应性患病率从7岁时的26%升至11岁时的31.6%。发现支气管对乙酰甲胆碱的反应性与特应性之间存在高度显著关联，这与症状或年龄组无关。
