Kim Min-Jeong, Park Sunhyun, Lee Ran-Sook, Lim Sang-Dong, Kim Hyo Jin, Lee Myung-Ki
Korea Food Research Institute, Seongnam 463-746, Korea.
Korean J Food Sci Anim Resour. 2014;34(1):65-72. doi: 10.5851/kosfa.2014.34.1.65. Epub 2014 Feb 28.
This study is executed to develop probiotics which produce S-adenosyl-L-methionine (SAM), a methyl group donor of the 5-methyltetrahydrofolate methylation reaction within the animal cell. SAM is an essential substance for the synthesis, activation, and metabolism of hormones, neurotransmitters, nucleic acids, phospholipids, and cell membranes of animals. The SAM is also known as a nutritional supplement to improve brain functions of the human. In this study, the SAM-producing strains are identified in 18 types of salted fish, and then, the strains with excellent SAM productions are being identified, with 1 strain in the Enterococcus genus and 9 strains in the Bacillus genus. Strains with a large amount of SAM production include the lactic acid bacteria such as En. faecium and En. durans, En. sanguinicola, as well as various strains in the Bacil-lus genus. The SAM-overproducing strains show antibacterial activities with certain harmful microbes in addition to the weak acid resistances and strong bile resistances, indicating characteristics of probiotics. It is possible that the jeotgal-originated beneficial strains with overproducing SAM can be commercially utilized in order to manufacture SAM enriched foods.
本研究旨在开发能够产生S-腺苷-L-甲硫氨酸（SAM）的益生菌，SAM是动物细胞内5-甲基四氢叶酸甲基化反应的甲基供体。SAM是动物激素、神经递质、核酸、磷脂和细胞膜合成、激活及代谢所必需的物质。SAM也作为一种营养补充剂，用于改善人类的脑功能。在本研究中，从18种咸鱼中鉴定出了产SAM的菌株，随后又鉴定出了产SAM能力优异的菌株，其中肠球菌属1株，芽孢杆菌属9株。大量产SAM的菌株包括屎肠球菌、耐久肠球菌、血肠球菌等乳酸菌以及芽孢杆菌属的各种菌株。产SAM过量的菌株除具有弱酸抗性和强胆汁抗性外，还对某些有害微生物表现出抗菌活性，显示出益生菌的特性。有可能将源自腌鱼的高产SAM有益菌株商业化用于生产富含SAM的食品。
