纳米比亚沙漠盐沼的宏病毒组学：嗜盐古菌盐沼噬菌体的一个新谱系以及单链DNA病毒的丰富来源。
Metaviromics of Namib Desert Salt Pans: A Novel Lineage of Haloarchaeal Salterproviruses and a Rich Source of ssDNA Viruses.
作者信息
Adriaenssens Evelien M, van Zyl Leonardo Joaquim, Cowan Don A, Trindade Marla I
机构信息
Centre for Microbial Ecology and Genomics, Genomics Research Institute, University of Pretoria, Natural Sciences II, Lynnwood Road, 0002 Pretoria, South Africa.
Institute for Microbial Biotechnology and Metagenomics, University of the Western Cape, 7535 Bellville, Cape Town, South Africa.
出版信息
Viruses. 2016 Jan 8;8(1):14. doi: 10.3390/v8010014.
Viral communities of two different salt pans located in the Namib Desert, Hosabes and Eisfeld, were investigated using a combination of multiple displacement amplification of metaviromic DNA and deep sequencing, and provided comprehensive sequence data on both ssDNA and dsDNA viral community structures. Read and contig annotations through online pipelines showed that the salt pans harbored largely unknown viral communities. Through network analysis, we were able to assign a large portion of the unknown reads to a diverse group of ssDNA viruses. Contigs belonging to the subfamily Gokushovirinae were common in both environmental datasets. Analysis of haloarchaeal virus contigs revealed the presence of three contigs distantly related with His1, indicating a possible new lineage of salterproviruses in the Hosabes playa. Based on viral richness and read mapping analyses, the salt pan metaviromes were novel and most closely related to each other while showing a low degree of overlap with other environmental viromes.
利用宏病毒组DNA的多重置换扩增和深度测序相结合的方法，对纳米比亚沙漠中两个不同盐田——霍萨贝斯盐田和艾斯费尔德盐田的病毒群落进行了调查，并提供了单链DNA和双链DNA病毒群落结构的全面序列数据。通过在线流程进行的读段和重叠群注释表明，盐田中存在大量未知的病毒群落。通过网络分析，我们能够将很大一部分未知读段归为一组多样的单链DNA病毒。属于嗜盐古菌病毒亚科的重叠群在两个环境数据集中都很常见。对嗜盐古菌病毒重叠群的分析显示，存在三个与His1远缘相关的重叠群，这表明在霍萨贝斯盐沼可能存在盐沼前病毒的一个新谱系。基于病毒丰富度和读段映射分析，盐田宏病毒组是新颖的，彼此之间关系最为密切，而与其他环境病毒组的重叠程度较低。
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