Olonade Israel, van Zyl Leonardo Joaquim, Trindade Marla
Institute for Microbial Biotechnology and Metagenomics (IMBM), University of the Western Cape, Bellville, Cape Town 7535, South Africa.
Microorganisms. 2021 Sep 28;9(10):2043. doi: 10.3390/microorganisms9102043.
Recent years have seen the classification and reclassification of many viruses related to the model enterobacterial phage P2. Here, we report the identification of a prophage (Smhb1) that infects BNH isolated from a Namib Desert salt pan (playa). Analysis of the genome revealed that it showed the greatest similarity to P2-like phages that infect species and showed no relation to any of the previously described -infecting phages. Despite being distantly related to these Vibrio infecting phages and sharing the same modular gene arrangement as seen in most P2-like viruses, the nucleotide identity to its closest relatives suggest that, for now, Smhb1 is the lone member of the genus . Although host range testing was not extensive and no secondary host could be identified for Smhb1, genomic evidence suggests that the phage is capable of infecting other species, including DV isolated from the same playa. Taken together, the analysis presented here demonstrates how adaptable the P2 phage model can be.
近年来，许多与典型肠道细菌噬菌体P2相关的病毒进行了分类和重新分类。在此，我们报告鉴定出一种前噬菌体（Smhb1），它感染从纳米比亚沙漠盐滩（盐碱滩）分离出的BNH。对其基因组的分析表明，它与感染弧菌属物种的P2样噬菌体具有最大的相似性，且与任何先前描述的感染弧菌属的噬菌体均无关联。尽管与这些感染弧菌属的噬菌体亲缘关系较远，并且与大多数P2样病毒具有相同的模块化基因排列，但其与其最亲近亲属的核苷酸同一性表明，目前Smhb1是弧菌噬菌体属的唯一成员。尽管宿主范围测试并不全面，且未为Smhb1鉴定出第二宿主，但基因组证据表明该噬菌体能够感染其他弧菌属物种，包括从同一盐碱滩分离出的弧菌DV。综上所述，此处给出的分析证明了P2噬菌体模型具有多么强的适应性。
