Deisenhofer J, Michel H
Howard Hughes Medical Institute, University of Texas Southwestern Medical Center, Dallas 75235.
EMBO J. 1989 Aug;8(8):2149-70. doi: 10.1002/j.1460-2075.1989.tb08338.x.
In our lectures we first describe the history and methods of membrane protein crystallization, before we show how the structure of the photosynthetic reaction centre from the purple bacterium Rhodopseudomonas viridis was solved. Then the structure of this membrane protein complex is correlated with its function as a light-driven electron pump across the photosynthetic membrane. Finally we draw conclusions on the structure of the photosystem II reaction centre from plants and discuss the aspects of membrane protein structure. Sections 1 (crystallization), 4 (conclusions on the structure of photosystem II reaction centre and evolutionary aspects) and 5 (aspects of membrane protein structure) were presented and written by H.M., Sections 2 (determination of the structure) and 3 (structure and function) by J.D. We have arranged the paper in this way in order to facilitate continuous reading.
在我们的讲座中，我们首先描述膜蛋白结晶的历史和方法，然后展示如何解析来自绿硫红假单胞菌的光合反应中心的结构。接着，将这种膜蛋白复合物的结构与其作为光合膜上光驱动电子泵的功能相关联。最后，我们得出关于植物光系统II反应中心结构的结论，并讨论膜蛋白结构的相关方面。第1节（结晶）、第4节（关于光系统II反应中心结构及进化方面的结论）和第5节（膜蛋白结构方面）由H.M.撰写并展示，第2节（结构测定）和第3节（结构与功能）由J.D.撰写。我们以这种方式编排论文，以便于连续阅读。
