Jiang Yun-bin, Gou Yan, Yuan Mao-hua, Ma Yu-ying, Zhou Juan, Wu Pi-e
Zhong Yao Cai. 2015 May;38(5):957-61.
To establish an HPLC fingerprint of Anisodus tanguticus root for its quality control.
The analysis was carried out on a Ultimate AQ C18 (250 mm x 4.6 mm, 5 μm) column with the gradient elution of acetonitrile and KH2PO4 buffer soution, whose pH was adjusted to 3.0 with phosphoric acid. The flow rate, column temperature, detection wavelength and injection volume was 1.0 mL/min, 30 degrees C, 210 nm and 10 μL separately. The similarity evaluation and principal component analysis were used to analyze HPLC fingerprint of Anisodus tanguticus root.
HPLC fingerprint of Anisodus tanguticus root was established with 15 common peaks by determining 18 batches of Anisodus tanguticus root samples. Four characteristic peaks, anisodine, scopolamine, anisodamine and anisodamine, were confirmed by comparing their retention time and UV spectrum with standard reference substances. The simiarities of 18 batches of Anisodus tanguticus root were between -0.891 and 0.987. Comprehensive evaluation scores of 18 batches of Anisodus tanguticus root were between -0.85 and 0.89 by principal component analysis.
The established HPLC fingerprint has good precision, repeatability and stability, which can provide more comprehensive information for identification and quality control of Anisodus tanguticus root.
建立唐古特山莨菪根的高效液相色谱指纹图谱以用于其质量控制。
采用Ultimate AQ C18（250 mm×4.6 mm，5μm）色谱柱，以乙腈和用磷酸调pH至3.0的KH2PO4缓冲溶液进行梯度洗脱。流速、柱温、检测波长和进样量分别为1.0 mL/min、30℃、210 nm和10μL。采用相似度评价和主成分分析对唐古特山莨菪根的高效液相色谱指纹图谱进行分析。
通过测定18批唐古特山莨菪根样品，建立了唐古特山莨菪根的高效液相色谱指纹图谱，共有15个共有峰。通过与对照品比较保留时间和紫外光谱，确认了4个特征峰，分别为樟柳碱、东莨菪碱、山莨菪碱和樟柳碱。18批唐古特山莨菪根的相似度在0.891至0.987之间。通过主成分分析，18批唐古特山莨菪根的综合评价得分在-0.85至0.89之间。
所建立的高效液相色谱指纹图谱具有良好准确性、重复性和稳定性，可以为唐古特山莨菪根的鉴别和质量控制提供更全面的信息。
