[贵州何首乌的高效液相指纹图谱]
[HPLC fingerprint chromatogram of Polygonum multiflorum from Guizhou].
作者信息
Li Yan, Wang Hui-Juan, Lin Bing, Zhao Zhi, Zhou Ying
机构信息
Guizhou Institute of Technology, Guiyang 550003, China.
出版信息
Zhong Yao Cai. 2012 Dec;35(12):1928-32.
OBJECTIVE
To establish the fingerprint of Polygonum multiflorum from Guizhou and provide a standard for its quality control.
METHODS
HPLC analysis was performed on Agillent ZABAX-C18 (4.6 mm x 250 mm, 5 microm), gradient eluted composed of acetonitrile-0.4% water solution of phosphoric acid. Column temperature was set at 25 degrees C and the flow rate was 1 mL/min. The detection wavelength was 280 nm and the analysis time was 60 min.
RESULTS
9 common peaks were identified. The RSD of the relative retention time and the relative peak area were less than 3% in analyzing its precision, stability and repeatability of the common peaks, and the similarity of the 16 batches of sample was more than 0.9.
CONCLUSION
The method is simple and reliable, and it can provide a standard and guidance for quality control of Polygonum multiflorum.
目的
建立贵州何首乌的指纹图谱，为其质量控制提供标准。
方法
采用高效液相色谱法，在Agillent ZABAX-C18（4.6 mm×250 mm，5微米）色谱柱上，以乙腈-0.4%磷酸水溶液进行梯度洗脱。柱温设定为25℃，流速为1 mL/min。检测波长为280 nm，分析时间为60 min。
结果
确定了9个共有峰。在分析共有峰的精密度、稳定性和重复性时，相对保留时间和相对峰面积的RSD均小于3%，16批样品的相似度均大于0.9。
结论
该方法简便可靠，可为何首乌的质量控制提供标准和指导。
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