Yubero Dèlia, Montero Raquel, Ramos Maria, Neergheen Viruna, Navas Plácido, Artuch Rafael, Hargreaves Iain
Clinical Biochemistry Department, Institut d'Investigació Sanitària Sant Joan De Déu and CIBERER, Barcelona, Spain.
Nephrology Department, Hospital Sant Joan De Déu, Barcelona, Spain.
Biofactors. 2015 Nov-Dec;41(6):424-30. doi: 10.1002/biof.1242. Epub 2015 Nov 19.
Kidney dysfunction is being increasingly associated with mitochondrial diseases and coenzyme Q10 (CoQ) deficiency. The assessment of CoQ status requires the biochemical determination of CoQ in biological fluids and different cell types, but no methods have been developed as yet for the analysis of CoQ in excretory systems. The aim of this study was to standardize a new procedure for urinary CoQ determination and to establish reference values for a paediatric population. Urinary CoQ was analyzed by HPLC with electrochemical detection. Reference values (n = 43) were stratified into two age groups (2-10 years: range 24-109 nmol CoQ/gram of pellet protein; 11-17 years: range 43-139 nmol CoQ/gram of pellet protein). In conclusion, urinary CoQ analysis is a noninvasive, reliable, and reproducible method to determine urinary tract CoQ status.
肾功能障碍与线粒体疾病和辅酶Q10（CoQ）缺乏的关联日益密切。评估CoQ状态需要对生物体液和不同细胞类型中的CoQ进行生化测定，但目前尚未开发出用于分析排泄系统中CoQ的方法。本研究的目的是规范一种测定尿CoQ的新方法，并建立儿科人群的参考值。采用高效液相色谱-电化学检测法分析尿CoQ。参考值（n = 43）分为两个年龄组（2 - 10岁：24 - 109 nmol CoQ/克沉淀蛋白；11 - 17岁：43 - 139 nmol CoQ/克沉淀蛋白）。总之，尿CoQ分析是一种测定尿路CoQ状态的非侵入性、可靠且可重复的方法。
