Reibarkh Mikhail, Wyche Thomas P, Saurí Josep, Bugni Tim S, Martin Gary E, Williamson R Thomas
Magn Reson Chem. 2015 Dec;53(12):i. doi: 10.1002/mrc.4384.
Utilization of isotopically labeled proteins and peptides is a routinely employed approach in biomolecular NMR investigations. The widespread availability of inexpensive, uniformly (13) C-enriched glucose now makes it possible to produce uniformly (13) C-labeled natural products by microbial fermentation. In this feature article, the authors describe an experimental approach for the rapid structural characterization of uniformly (13) C-labeled natural products based on the Constant-Time HSQC (CT-HSQC) experiment. Rigorous theoretical evaluation of the CT-HSQC experiment allowed the applicability of the experiment to be expanded from the traditional, narrow scope of labeled amino acids to encompass virtually any small molecule or U-(13) C labeled natural product. A suite of experiments including CT-HSQC, (13) C-(13) C COSY, and COSYLR experiments is sufficient for the structure elucidation of uniformly (13) C-labeled small molecules and natural products. Differences in NMR approaches for structure elucidation of natural abundance and uniformly (13) C-labeled molecules are also discussed. The present work provides a researcher working in this area of natural products chemistry with NMR structure elucidation tools for investigating (13) C-labeled small molecules and natural products.
在生物分子核磁共振研究中，使用同位素标记的蛋白质和肽是一种常规采用的方法。现在，廉价的、均匀富集¹³C的葡萄糖广泛可得，这使得通过微生物发酵生产均匀¹³C标记的天然产物成为可能。在这篇专题文章中，作者描述了一种基于恒时HSQC（CT-HSQC）实验对均匀¹³C标记的天然产物进行快速结构表征的实验方法。对CT-HSQC实验进行的严格理论评估，使该实验的适用性从传统的、标记氨基酸的狭窄范围扩展到几乎涵盖任何小分子或均匀¹³C标记的天然产物。一套包括CT-HSQC、¹³C-¹³C COSY和COSYLR实验在内的实验足以用于阐明均匀¹³C标记的小分子和天然产物的结构。文中还讨论了天然丰度分子和均匀¹³C标记分子在结构阐明方面核磁共振方法的差异。目前的工作为从事天然产物化学这一领域研究的人员提供了用于研究¹³C标记的小分子和天然产物的核磁共振结构阐明工具。
