Gloghini A, Colombatti A, Bressan G, Carbone A
Division of Pathology, Centro di Riferimento Oncologico, Aviano, Italy.
Hum Pathol. 1989 Oct;20(10):1001-7. doi: 10.1016/0046-8177(89)90272-4.
We analyzed the distribution of two basement membrane components, laminin and type IV collagen, in human reactive and neoplastic lymphoid follicles by using immunoenzymatic and immunofluorescence methods on serial frozen sections from 11 reactive lymph nodes, three palatine tonsils, and 11 immunophenotypically defined B cell non-Hodgkin's lymphomas. The patterns of distribution of these two antigens were compared with that of follicular dendritic reticulum cells (DRCs) as visualized by anti-DRC-1 and anti-desmoplakin 1 and 2 antibodies. Immunostaining for laminin and type IV collagen produced overlapping results. Germinal centers contained some vascular and fiber-like linear staining, but the most striking feature was the presence of a large amount of punctate-granular staining. This staining was present throughout the entire area of most of the germinal centers, although often more densely in the central areas. The stronger punctate-granular staining for laminin, type IV collagen, and desmoplakin 1 and 2 corresponded to the more densely stained areas for DRC-1. Double immunofluorescence assay demonstrated that there was a close similarity between the punctate-granular staining pattern of laminin and the dendritic network of DRC-1-positive cells. In the nine B cell lymphomas of follicular center origin in which DRCs were present, although distributed in different patterns, immunostaining for laminin and type IV collagen could be observed only in those areas showing immunoreactivity for DRC-1. The results of this study suggest that the punctate-granular staining pattern for laminin and type IV collagen is specific for the follicle. This finding may be strictly related to the follicular microenvironment because the pattern of distribution displayed by laminin and type IV collagen seems to be spatially related to the staining pattern of DRCs, as visualized by the anti-DRC-1 and desmoplakins antibodies, both in reactive and neoplastic conditions.
我们采用免疫酶法和免疫荧光法，对11个反应性淋巴结、3个腭扁桃体以及11例免疫表型明确的B细胞非霍奇金淋巴瘤的连续冰冻切片进行分析，研究层粘连蛋白和IV型胶原这两种基底膜成分在人类反应性和肿瘤性淋巴滤泡中的分布情况。将这两种抗原的分布模式与通过抗DRC-1抗体以及抗桥粒斑蛋白1和2抗体所显示的滤泡树突状网状细胞（DRC）的分布模式进行比较。层粘连蛋白和IV型胶原的免疫染色结果相互重叠。生发中心有一些血管样和纤维样线性染色，但最显著的特征是存在大量点状颗粒状染色。这种染色在大多数生发中心的整个区域都有，不过在中心区域通常更为密集。层粘连蛋白、IV型胶原以及桥粒斑蛋白1和2的点状颗粒状染色越强，对应的DRC-1染色区域就越密集。双重免疫荧光检测表明，层粘连蛋白的点状颗粒状染色模式与DRC-1阳性细胞的树突状网络之间存在密切相似性。在存在DRC的9例滤泡中心来源的B细胞淋巴瘤中，尽管DRC分布模式各异，但仅在那些对DRC-1呈免疫反应的区域能观察到层粘连蛋白和IV型胶原的免疫染色。本研究结果表明，层粘连蛋白和IV型胶原的点状颗粒状染色模式是滤泡特有的。这一发现可能与滤泡微环境密切相关，因为在反应性和肿瘤性情况下，层粘连蛋白和IV型胶原所呈现的分布模式似乎在空间上与通过抗DRC-1抗体和桥粒斑蛋白抗体所显示的DRC染色模式相关。
