Aras Yavuz, Sabanci Pulat Akin, Kabatas Serdar, Duruksu Gokhan, Subasi Cansu, Erguven Mine, Karaoz Erdal
Istanbul University, Istanbul School of Medicine, Department of Neurosurgery, Istanbul, Turkey.
Turk Neurosurg. 2016;26(1):127-39. doi: 10.5137/1019-5149.JTN.15724-15.0.
To investigate the effectiveness of rat adipose tissue-derived (rAT) mesenchymal stem cell (MSC) transplantation on the functional restoration and regeneration of spinal cord injury (SCI).
Six of 48 Wistar albino rats were sacrificed to obtain MSCs, and the remaining rats were divided randomly into six groups. SCI was performed using the clip compression method. The control and transplantation groups were injected with physiological saline and a rAT-MSC suspension at the injury sites, respectively. Each animal was evaluated using the Basso, Beattie and Bresnahan (BBB) rating system and sacrificed at 28 days post-injury period (p.i.). The regeneration process was evaluated based on immunostaining against β3-tubulin, BDNF, CNTF, and CNPase.
rAT-MSC transplantation into the SCI site substantially improved the tissue regeneration and functional recovery (p < 0.05). However, the rAT-MSC transplantation at 9 days p.i. was not more efficient on functional recovery than the transplantation immediately after injury. The expression of β3-tubulin, BDNF and CNTF at the injury site indicated the potential for functional regeneration.
The adaptive nature of rat-MSCs enabled the remodulation and regeneration of the lesion site, decreasing the importance of transplantation time in the treatment of SCI.
探讨大鼠脂肪组织来源的（rAT）间充质干细胞（MSC）移植对脊髓损伤（SCI）功能恢复和再生的有效性。
处死48只Wistar白化大鼠中的6只以获取间充质干细胞，其余大鼠随机分为6组。采用夹压法造成脊髓损伤。对照组和移植组分别在损伤部位注射生理盐水和rAT-MSC悬液。每只动物均采用Basso、Beattie和Bresnahan（BBB）评分系统进行评估，并在损伤后28天处死。基于针对β3微管蛋白、脑源性神经营养因子（BDNF）、睫状神经营养因子（CNTF）和2',3'-环核苷酸3'-磷酸二酯酶（CNPase）的免疫染色评估再生过程。
将rAT-MSC移植到脊髓损伤部位可显著改善组织再生和功能恢复（p < 0.05）。然而，损伤后9天进行rAT-MSC移植在功能恢复方面并不比损伤后立即移植更有效。损伤部位β3微管蛋白、BDNF和CNTF的表达表明具有功能再生的潜力。
大鼠间充质干细胞的适应性使其能够对损伤部位进行重塑和再生，降低了移植时间在脊髓损伤治疗中的重要性。
