Neurosciences Research Center (NSRC), Tabriz University of Medical Sciences, Tabriz, Iran.
Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Life Sci. 2019 Mar 15;221:20-28. doi: 10.1016/j.lfs.2019.02.011. Epub 2019 Feb 5.
In the most of previous experiments, intrathecal administration of stem cells (SCs) was seen in the management of neurogenic bladder (NGB) following contusion or complete transaction in the rodent model of spinal cord injury (SCI). Here, we aimed to investigate whether intra bladder wall autologous bone marrow mesenchymal SC (BM-MSCs) transplantation, as a minimally invasive method, could improve bladder dysfunctions after a chronic phase of hemi- and complete-transection SCI in a female rat model.
A total of forty-two female Wistar rats were randomly divided into 6 groups (each in 7) and subjected to complete and incomplete spinal cord transection by a laminectomy at the T9 vertebral level. Four weeks after SCI operation, BM-MSCs (1 × 10/120 μl) were transplanted in six areas of the bladder muscle in rats with complete SCI (cSCI) and hemi SCI (hSCI) groups. In the rats from sham, cSCI and hSCI negative control groups, normal saline was injected instead of BM-MSCs. Four weeks post-cell transplantation, rats were subjected to conscious urodynamic for voiding function assessment.
All bladders in cSCI and hSCI groups were the hyperreflexic type. The amplitude of uninhibited contraction in cSCI + BM-MSC group was decreased (p = 0.046). we noted that compliance was recovered in the hSCI + BM-MSCs group (p = 0.041). Residual volume was increased significantly after SCI while cell transplantation decreased this index in both hSCI and cSCI +BM-MSCs groups. The statistically significant result was only seen in the hSCI group (p = 0.046). Data showed that collagen deposition was markedly increased in the SCI group compared to the control or sham groups. These changes were decreased post-treatment in the hSCI group (p = 0.042).
Our study added a notion that urinary dysfunction associated with SCI, was improved following direct injection of autologous BM-MSC transplantation to bladder wall in the chronic phase of SCI injury.
在大多数先前的实验中，鞘内给予干细胞（SCs）被认为是在啮齿动物脊髓损伤（SCI）模型中挫伤或完全横断后治疗神经源性膀胱（NGB）的一种方法。在这里，我们旨在研究膀胱壁内自体骨髓间充质干细胞（BM-MSCs）移植是否可以改善半横断和完全横断 SCI 后慢性期雌性大鼠模型的膀胱功能障碍。
总共 42 只雌性 Wistar 大鼠被随机分为 6 组（每组 7 只），通过椎板切除术在 T9 椎体水平进行完全和不完全脊髓横断。SCI 手术后 4 周，将 BM-MSCs（1×10/120μl）注入完全 SCI（cSCI）和半 SCI（hSCI）组大鼠膀胱肌肉的 6 个区域。在假手术、cSCI 和 hSCI 阴性对照组的大鼠中，注射生理盐水代替 BM-MSCs。细胞移植后 4 周，对大鼠进行清醒尿动力学评估排尿功能。
cSCI 和 hSCI 组的所有膀胱均为反射亢进型。cSCI+BM-MSC 组无抑制收缩幅度降低（p=0.046）。我们注意到 hSCI+BM-MSCs 组的顺应性得到恢复（p=0.041）。SCI 后残余尿量显著增加，而细胞移植可降低 hSCI 和 cSCI+BM-MSCs 组的这一指标。hSCI 组的结果具有统计学意义（p=0.046）。数据显示，与对照组或假手术组相比，SCI 组胶原沉积明显增加。hSCI 组治疗后这些变化减少（p=0.042）。
我们的研究增加了一种观点，即与 SCI 相关的尿功能障碍，在 SCI 损伤的慢性期通过直接注射自体 BM-MSC 移植到膀胱壁可以得到改善。
